Application
Western blot analysis of MT-MMP-1 expression in non-transfected: (A) and human MT-MMP-1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of MT-MMP-1 expression in MIA PaCa-2 whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human oral mucosa tissue showing membrane and cytoplasmic staining of surface epithelial cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.| Product Name | MT-MMP-1 Antibody (H-72) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 511-582 mapping at the C-terminus of MT-MMP-1 of human origin |
| Modification Notes | The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan (1). Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB) (2). MMP catalysis requires both calcium and zinc (3). Membrane-type matrix metalloproteinases, including MT-MMP-1 (also designated MMP-14), MT-MMP-2 (also designated MMP-15), MT-MMP-3 (also designated MMP-16) and MT-MMP-4 (also designated MMP-17) are type I membrane proteins that function to activate other MMPs (4,5). MT-MMP activation appears to be mediated by members of the proprotein convertase family, suggesting that a proprotein convertase/MT-MMP/MMP cascade may be involved in the regulation of ECM turnover (6,7). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of MT-MMP-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 511-582 mapping at the C-terminus of MT-MMP-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 511-582 mapping at the C-terminus of MT-MMP-1 of human origin |
| Entrez Gene | 4323 |
|---|
Application
Western blot analysis of MT-MMP-1 expression in non-transfected: (A) and human MT-MMP-1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of MT-MMP-1 expression in MIA PaCa-2 whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human oral mucosa tissue showing membrane and cytoplasmic staining of surface epithelial cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.| Application Notes | recommended for detection of MT-MMP-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
