Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in tubules.Application
Western blot analysis of Bim expression in non-transfected: (A) and mouse Bim transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of normal mouse lymph node frozen section showing cytoplasmic staining.Application
Western blot analysis of Bim expression in non-transfected control (A) and Bim siRNA transfected (B) HeLa cells. Blot probed with Bim (H-191): . emerin (H-12): used as specificity and loading control.Application
Western blot analysis of Bim isoform expression in HuT 78 whole cell lysate.| Product Name | Bim Antibody (H-191) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 4-195 of BimEL of human origin |
| Modification Notes | Pro-apototic Bcl-2 family members promote cell death by neutralizing their anti-apoptotic relatives, which otherwise maintain cell viability by regulating caspase activity. Bim belongs to the BH3-only subgroup of Bcl-2 related proteins, and exists in three distinct isoforms, BimS (short), BimL (long) and BimEL (extra long). ERK1/2 phosphorylates BimEL, resulting in rapid degradation of the isoform via the proteasome pathway. At least three sites for ERK1/2 phosphorylation exist on BimEL, whereas ERK1/2 does not effect BimS and BimL, implying a unique role for BimEL in cell survival signaling. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of BimEL, BimL and BimS of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 4-195 of BimEL of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 4-195 of BimEL of human origin |
| Entrez Gene | 10018 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in tubules.
Application
Western blot analysis of Bim expression in non-transfected: (A) and mouse Bim transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of normal mouse lymph node frozen section showing cytoplasmic staining.
Application
Western blot analysis of Bim expression in non-transfected control (A) and Bim siRNA transfected (B) HeLa cells. Blot probed with Bim (H-191): . emerin (H-12): used as specificity and loading control.
Application
Western blot analysis of Bim isoform expression in HuT 78 whole cell lysate.| Application Notes | recommended for detection of BimEL, BimL and BimS of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
