Application
Western blot analysis of EWS expression in non-transfected: (A) and human EWS transfected: (B) 293T whole cell lysates.Application
Western blot analysis of EWS expression in non-transfected: (A) and human EWS transfected: (B) 293T whole cell lysates.Application
Western blot analysis of EWS expression in HeLa whole cell lysate.Application
Western blot analysis of EWS expression in non-transfected 293: (A), human EWS transfected 293: (B) and Jurkat (C) whole cell lysates.| Product Name | EWS Antibody (H-60) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | EWS is a nuclear RNA-binding protein. As a result of chromosome translocation, the EWS gene is fused to a variety of transcription factors, including ATF-1 in human neoplasias. In the Ewing family of tumors, the N-terminal domain of EWS is fused to the DNA-binding domain of various ETS transcription factors, including Fli-1, Erg, ETV1, E1AF and FEV. The EWS/Fli-1 chimeric protein acts as a more potent transcriptional activator than Fli-1 and can promote cell transformation. Two functional regions have been identified in EWS; an amino-terminal region (domain A), that has little transactivation activity but transforms efficiently when fused to Fli-1, and a distal region (domain B), which shows transactivation activity but transforms less efficiently when fused to Fli-1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Ewing sarcoma breakpoint protein of mouse, rat and human origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 431-490 mapping within an internal region of EWS of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 431-490 mapping within an internal region of EWS of human origin |
| Entrez Gene | 2130 |
|---|
Application
Western blot analysis of EWS expression in non-transfected: (A) and human EWS transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of EWS expression in non-transfected: (A) and human EWS transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of EWS expression in HeLa whole cell lysate.
Application
Western blot analysis of EWS expression in non-transfected 293: (A), human EWS transfected 293: (B) and Jurkat (C) whole cell lysates.| Application Notes | recommended for detection of Ewing sarcoma breakpoint protein of mouse, rat and human origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
