Application
Western blot analysis of Bag-1 expression in HeLa whole cell lysate.Application
Western blot analysis of Bag-1 expression in non-transfected: (A) and human Bag-1 transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear localization.Application
Western blot analysis of Bag-1 expression in non-transfected control (A) and Bag-1 siRNA transfected (B) C2C12 cells. Blot probed with Bag-1 (FL-274): . Actin (I-19): used as specificity and loading control.Application
Western blot analysis of Bag-1 expression in c4 (A), RAW 264.7 (B) and Sol8 (C) whole cell lysates.Application
Western blot analysis of Bag-1 isoform expression in HeLa (A) and HL-60 (B) whole cell lysates and mouse testis extract (C).| Product Name | Bag-1 Antibody (FL-274) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The Bcl-2 family of proteins is characterized by its ability to modulate cell death (apoptosis) under a broad range of physiologic conditions. Bcl-2 and several related proteins function to inhibit apoptosis, while other members of the Bcl-2 family, such as Bax and Bak, enhance cell death under various conditions. For instance, Bcl-xL represses cell death, while its shorter form, Bcl-xS, promotes apoptosis. Dimerization of another member of this family, Bad, with Bcl-xL, results in displacement of Bax from Bcl-xL/Bax complexes and restoration of Bax-mediated apoptosis. A Bcl-2-binding protein, designated Bag-1, lacks significant homology with Bcl-2 or with other Bcl-2-related proteins. Bag-1 appears to function to enhance Bcl-2 protection from cell death, suggesting that Bag-1 represents a new type of anti-cell death gene. This also suggests that certain routes of apoptosis induction, previously ascribed to Bcl-2-independent pathways, may instead reflect a requirement for a combination of Bcl-2 and Bag-1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Bag-1 p32, p36 and p50 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 73-274 mapping at the C-terminus of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 73-274 mapping at the C-terminus of human origin |
| Entrez Gene | 573 |
|---|
Application
Western blot analysis of Bag-1 expression in HeLa whole cell lysate.
Application
Western blot analysis of Bag-1 expression in non-transfected: (A) and human Bag-1 transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear localization.
Application
Western blot analysis of Bag-1 expression in non-transfected control (A) and Bag-1 siRNA transfected (B) C2C12 cells. Blot probed with Bag-1 (FL-274): . Actin (I-19): used as specificity and loading control.
Application
Western blot analysis of Bag-1 expression in c4 (A), RAW 264.7 (B) and Sol8 (C) whole cell lysates.
Application
Western blot analysis of Bag-1 isoform expression in HeLa (A) and HL-60 (B) whole cell lysates and mouse testis extract (C).| Application Notes | recommended for detection of Bag-1 p32, p36 and p50 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
