Application
Western blot analysis of human recombinant Cytokeratin 12 fusion protein.| Product Name | Cytokeratin 12 Antibody (H-60) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue, where they constitute up to 85% of mature keratinocytes in the vertebrate epidermis. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. The å-helical, coiled-coil dimers associate laterally end-to-end to form 10 nm diameter filaments. Cytokeratins are useful markers of tissue differentiation, and Cytokeratin 12 is a distinct marker of tissue differentiation in the developing cornea. Cytokeratin 12 and Cytokeratin 3 are expressed in the corneal epithelium, where Cytokeratin 12 provides structural integrity to an otherwise fragile cornea. Human Cytokeratin 12 gene mutations cause Meesmann’s corneal dystrophy, an autosomal dominant disorder characterized by corneal epithelia fragility and intra-epithelial microcysts. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Cytokeratin 12 of human origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-60 of Cytokeratin 12 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-60 of Cytokeratin 12 of human origin |
| Entrez Gene | 3859 |
|---|
Application
Western blot analysis of human recombinant Cytokeratin 12 fusion protein.| Application Notes | recommended for detection of Cytokeratin 12 of human origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
