Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of eIF2Bε expression in non-transfected 293T: (A), human eIF2Bε transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of eIF2Bε expression in non-transfected: (A) and mouse eIF2Bε transfected: (B) 293T whole cell lysates.Application
Western blot analysis of eIF2Bε expression in non-transfected: (A) and mouse eIF2Bε transfected: (B) 293T whole cell lysates.Application
Western blot analysis of eIF2Bε expression in KNRK whole cell lysate.Application
Western blot analysis of eIF2Bε expression in HeLa whole cell lysate.| Product Name | eIF2Bε Antibody (H-290) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex eIF2B exists as a five subunit complex composed of eIF2Bå, eIF2Bß, eIF2B©, eIF2B∂, and eIF2Bé. The eIF2B complex catalyzes the exchange of GDP for GTP on the eIF2 complex, following the interaction of eIF2/GTP with the 40S ribosomal subunit. Guanine nucleotide exchange factor (GEF) activity is exhibited by the eIF2Bé subunit alone, but is greater in the presence of all five eIF2B subunits. Phosphorylation of eIF2 inhibits GEF activity of eIF2B, an inhibition that requires the eIF2Bå subunit. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of eIF2B&epsilon of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 422-711 mapping near the C-terminus of eIF2B&epsilon of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 422-711 mapping near the C-terminus of eIF2Bε of human origin |
| Entrez Gene | 8893 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of eIF2Bε expression in non-transfected 293T: (A), human eIF2Bε transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of eIF2Bε expression in non-transfected: (A) and mouse eIF2Bε transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of eIF2Bε expression in non-transfected: (A) and mouse eIF2Bε transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of eIF2Bε expression in KNRK whole cell lysate.
Application
Western blot analysis of eIF2Bε expression in HeLa whole cell lysate.| Application Notes | recommended for detection of eIF2B&epsilon of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
