Application
Western blot analysis of AK3 expression in non-transfected: (A) and mouse AK3 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of AK3 expression in non-transfected: (A) and human AK3 transfected: (B) 293T whole cell lysates.| Product Name | AK3 Antibody (H-45) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 41-85 mapping near the N-terminus of AK3 of human origin |
| Modification Notes | Adenylate kinases 1-5 (designated AK1-5) are a set of enzymes that regulate the phosphorylation state of intracellular adenine nucleotides, which are the principle high-energy phosphoryl-carrying molecules in living cells. By catalyzing phosphoryl transfer between adenine nucleotides (AMP, ADP, ATP), AKs influence metabolic signals, which include gene expression, ion channel activity and protein kinase-mediated signaling. Inherited mutations leading to AK deficiencies in erythrocytes have been implicated in hemolytic anemia. Human AK3 is a 223 amino acid protein that is present in the mitochondria of liver and heart. It utilizes GTP as a substrate relative to isoforms AK1 and AK2, which use ATP. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of AK3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 41-85 mapping near the N-terminus of AK3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 41-85 mapping near the N-terminus of AK3 of human origin |
| Entrez Gene | 50808 |
|---|
Application
Western blot analysis of AK3 expression in non-transfected: (A) and mouse AK3 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of AK3 expression in non-transfected: (A) and human AK3 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of AK3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
