Application
Western blot analysis of 3pK expression in non-transfected: (A) and human 3pK transfected: (B) 293T whole cell lysates.Application
Western blot analysis of 3pK expression in HeLa whole cell lysate.Application
Immunofluorescence staining of normal mouse intestine frozen section showing perinuclear staining.| Product Name | 3pK Antibody (H-50) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-50 mapping at the N-terminus of 3pK of human origin |
| Modification Notes | The MAPKAP kinases (for MAP kinase activated protein kinases) are a group of MAP kinase substrates which are themselves kinases. In response to activation, the MAP kinases phosphorylate downstream components on a consensus Pro-X-Ser/Thr-Pro motif. Several kinases that contain this motif have been identifed and serve as substrates for the ERK and p38 MAP kinases. These include the serine/threonine kinases Rsk-1 (also designated MAPKAP kinase-1), Rsk-2 and Rsk-3, which are phosphorylated by ERK1 and ERK2. Similarly, p38 phosphorylates and activates the serine/threonine kinases MAPKAP kinase-2 and MAPKAP kinase-3 (also designated 3pK). The serine/threonine kinases Mnk1 and Mnk2 are substrates for both ERK and p38 MAP kinases. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of 3pK of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-50 mapping at the N-terminus of 3pK of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-50 mapping at the N-terminus of 3pK of human origin |
| Entrez Gene | 7867 |
|---|
Application
Western blot analysis of 3pK expression in non-transfected: (A) and human 3pK transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of 3pK expression in HeLa whole cell lysate.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing perinuclear staining.| Application Notes | recommended for detection of 3pK of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
