Application
Western blot analysis of LEF-1 expression in non-transfected: (A) and human LEF-1 transfected: (B) 293T whole cell lysates.| Product Name | LEF-1 Antibody (H-70) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 61-130 mapping near the N-terminus of LEF-1 of human origin |
| Modification Notes | The TCF (T cell factor) family of transcription factors are activated by the Wnt-1 and Wingless pathways and are characterized by the presence of a conserved protein motif, the high mobility group (HMG) 1 box, which mediates DNA binding. The TCF (T cell factor) proteins, which are required during development, include TCF-1, which is essential for lymphoid cell development, and TCF-3 and TCF-4, which are implicated in neuronal development and LEF (leukemia enhancer factor).The Wnt mediated signaling pathway induces cytosolic ß-catenin binding to TCF proteins within the nucleus, leading to the enhanced expression of the Wnt target genes. The ß-catenin-TCF complexes are negatively regulated by the adenomatous polyposis coli (APC) tumor suppressor protein, which phosphorylates ß-catenin and, in turn, increases the degradation of cytosolic ß-catenin to, thereby, inhibit the transcriptional activity of the TCF proteins. Mutations in the APC gene, which are commonly observed in colorectal carcinomas, disrupt this regulatory pathway and correlate to an accumulation of ß-catenin and the increased activation of the TCF target genes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of LEF-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 61-130 mapping near the N-terminus of LEF-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 61-130 mapping near the N-terminus of LEF-1 of human origin |
| Entrez Gene | 51176 |
|---|
Application
Western blot analysis of LEF-1 expression in non-transfected: (A) and human LEF-1 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of LEF-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
