Application
Western blot analysis of Ran BP-1 expression in NIH/3T3 whole cell lysate.Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.Application
Western blot analysis of Ran BP-1 expression in NIH/3T3 (A) and F9 (B) whole cell lysates and mouse testis tissue extract (C).| Product Name | Ran BP-1 Antibody (M-45) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 159-203 mapping at the C-terminus of Ran BP-1 of mouse origin |
| Modification Notes | The small Ras-related protein Ran, also called TC4, is a nuclear localized GTPase implicated in a diverse array of cellular processes including DNA replication, entry into and exit from mitosis and the transport of RNA and proteins through the nuclear pore complex. Like Ras, active Ran GTP and inactive Ran GDP levels are tightly regulated by guanine nucleotide exchange factors (GEFs) and GTPase activating proteins (GAPs). The abundant GEF, RCC1 (regulator of chromosome condensation 1), increases the rate at which Ran exchanges GDP for GTP. Ran GAP1 opposes the effects of RCC1 by increasing the rate at which Ran hydrolyzes GTP to GDP. A protein designated Ran BP1 has no intrinsic GAP activity, and functions as a GEF inhibitor deactivating RCC1 and thereby indirectly increasing the ratio of Ran GDP to Ran GTP. The Ran BP2 protein has been proposed as the Ran GTP docking site at the periphery of the nuclear pore complex. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Ran BP-1 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 159-203 mapping at the C-terminus of Ran BP-1 of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 159-203 mapping at the C-terminus of Ran BP-1 of mouse origin |
| Entrez Gene | 5902 |
|---|
Application
Western blot analysis of Ran BP-1 expression in NIH/3T3 whole cell lysate.
Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.
Application
Western blot analysis of Ran BP-1 expression in NIH/3T3 (A) and F9 (B) whole cell lysates and mouse testis tissue extract (C).| Application Notes | recommended for detection of Ran BP-1 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
