Application
Western blot analysis of UCH-L3/4 expression in non-transfected: (A) and human UCH-L3/4 transfected: (B) 293 whole cell lysates.| Product Name | UCH-L3/4 Antibody (H-40) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 191-230 mapping at the C-terminus of UCH-L3 of human origin |
| Modification Notes | UCH-L1 (ubiquitin C-terminal hydrolase) is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCH-L1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. UCH-L1 is expressed in brain neurons. Examination of specific brain regions reveals expression in all areas tested, particularly in the substantia nigra. UCH-L1 represents 1 to 2% of total soluble brain protein. Its occurrence in Lewy bodies and its function in the proteasome pathway make it a compelling candidate gene in Parkinson disease. The gene which encodes UCH-L1 maps to human chromosome 4p14. The 230 amino acid human UCH-L3 protein is 54% identical to that of UCH-L1. UCH-L3 is the predominant thiol protease and has high-affinity binding sites for ubiquitin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of UCH-L3 of mouse, rat, and human origin and UCH-L4 of mouse origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 191-230 mapping at the C-terminus of UCH-L3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 191-230 mapping at the C-terminus of UCH-L3 of human origin |
| Entrez Gene | 50933 |
|---|
Application
Western blot analysis of UCH-L3/4 expression in non-transfected: (A) and human UCH-L3/4 transfected: (B) 293 whole cell lysates.| Application Notes | recommended for detection of UCH-L3 of mouse, rat, and human origin and UCH-L4 of mouse origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
