Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing cytoplasmic and nuclear staining of glandular cells and interstitial cells.Application
Western blot analysis of Cdk4 expression in Ramos whole cell lysate.Application
Note presence of Protein A/G band using Protein A/G PLUS-Agarose: conventional IP matrix (A) as compared to their absence using ExactaCruz A: (B).Application
Western blot analysis of Cdk4 expression in non-transfected 293T: (A), mouse Cdk4 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of Cdk4 expression in non-transfected control (A) and Cdk4 siRNA transfected (B) HeLa cells. Blot probed with Cdk4 (C-22): . Actin (I-19): used as specificity and loading control.Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoskeletal rhodamine immunostaining of myosin heavy chain. Note also nuclear rhodamine immunostaining with Cdk4 (C-22): .Application
Western blot analysis of Cdk4 p34 expression in HeLa whole cell lysate.Application
Western blot analysis of Cdk4 expression in NIH/3T3 nuclear extract.| Product Name | Cdk4 Antibody (C-22) HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of Cdk4 of mouse origin |
| Modification Notes | Cell cycle progression is controlled in part by a family of cyclin proteins and cyclin dependent kinases (Cdks). Cdk proteins work in concert with the cyclins to phosphorylate key substrates involved in each phase of cell cycle progression. Another family of proteins, Cdk inhibitors, also plays a role in regulating the cell cycle by binding to cyclin-Cdk complexes and modulating their activity. Several Cdk proteins have been identified, including Cdk2–Cdk8, PCTAIRE-1–3, PITALRE and PITSLRE. Cdk4, in complex with D-type cyclins, is thought to regulate cell growth during the G1 phase of the cell cycle. This association with a D-type cyclin upregulates Cdk4 activity, whereas binding to the Cdk inhibitor p16 downregulates Cdk4 activity. Activation of the Cdk4-cyclin complexes requires phosphorylation on a single threonyl residue of Cdk4, catalyzed by a Cdk-activating protein (CAK). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Cdk4 p34 of mouse, rat and human origin by WB, IP, IF, IHC(P), KA and ELISA also reactive with additional species, including bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of Cdk4 of mouse origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of Cdk4 of mouse origin |
| Entrez Gene | 1019 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing cytoplasmic and nuclear staining of glandular cells and interstitial cells.
Application
Western blot analysis of Cdk4 expression in Ramos whole cell lysate.
Application
Note presence of Protein A/G band using Protein A/G PLUS-Agarose: conventional IP matrix (A) as compared to their absence using ExactaCruz A: (B).
Application
Western blot analysis of Cdk4 expression in non-transfected 293T: (A), mouse Cdk4 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of Cdk4 expression in non-transfected control (A) and Cdk4 siRNA transfected (B) HeLa cells. Blot probed with Cdk4 (C-22): . Actin (I-19): used as specificity and loading control.
Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoskeletal rhodamine immunostaining of myosin heavy chain. Note also nuclear rhodamine immunostaining with Cdk4 (C-22): .
Application
Western blot analysis of Cdk4 p34 expression in HeLa whole cell lysate.
Application
Western blot analysis of Cdk4 expression in NIH/3T3 nuclear extract.| Application Notes | recommended for detection of Cdk4 p34 of mouse, rat and human origin by WB, IP, IF, IHC(P), KA and ELISA also reactive with additional species, including bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
