Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in tubules.Application
Western blot analysis of IKKγ expression in non-transfected 293: (A), human IKKγ transfected 293: (B) and Jurkat (C) whole cell lysates.Application
Western blot analysis of IKKγ expression in non-transfected: (A) and human IKKγ transfected: (B) 293T whole cell lysates.Application
Western blot analysis of IKKγ expression in non-transfected 293T: (A), mouse IKKγ transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of IKKγ expression in non-transfected: (A) and human IKKγ transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human tonsil tissue showing cytoplasmic staining of follicle and non-follicle cells and surface epithelial cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of IKKγ1 and IKKγ2 expression in BJAB (A), Jurkat (B) and K-562 (C) whole cell lysates.| Product Name | IKKγ Antibody (FL-419) TRITC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against full length IKKγ of human origin |
| Modification Notes | The transcription factor NFkB is retained in the cytoplasm in an inactive form by the inhibitory protein IkB. Activation of NFkB requires that IkB be phosphorylated on specific serine residues, which results in targeted degradation of IkB. IkB kinase å (IKKå), previously designated CHUK, interacts with IkB-å and specifically phosphorylates I˚Bå on Serine 32 and 36, the sites that trigger its degradation. IKKå appears to be critical for NFkB activation in response to proinflammatory cytokines. Phosphorylation of IkB by IKKå is stimulated by the NFkB inducing kinase (NIK), which itself is a central regulator for NFkB activation in response to TNF and IL-1. The functional IKK complex contains three subunits, IKKå, IKK∫ and IKK© (also designated NEMO), and each appear to make essential contributions to IkB phosphorylation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of IKKγ of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against full length IKK&gamma of human origin |
|---|---|
| Tissue Specificity | raised against full length IKKγ of human origin |
| Entrez Gene | 8517 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in tubules.
Application
Western blot analysis of IKKγ expression in non-transfected 293: (A), human IKKγ transfected 293: (B) and Jurkat (C) whole cell lysates.
Application
Western blot analysis of IKKγ expression in non-transfected: (A) and human IKKγ transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of IKKγ expression in non-transfected 293T: (A), mouse IKKγ transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of IKKγ expression in non-transfected: (A) and human IKKγ transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human tonsil tissue showing cytoplasmic staining of follicle and non-follicle cells and surface epithelial cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of IKKγ1 and IKKγ2 expression in BJAB (A), Jurkat (B) and K-562 (C) whole cell lysates.| Application Notes | recommended for detection of IKKγ of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
