Application
Western blot analysis of β Tubulin expression in A-431 (A), BJAB (B), NIH/3T3 (C) and KNRK (D) whole cell lysates.Application
Western blot analysis of β2A Tubulin expression in non-transfected 293T: (A), human β2A Tubulin transfected 293T: (B) and A-431 (C) whole cell lysates.Application
Western blot analysis of β2C Tubulin expression in non-transfected 293T: (A), mouse β2C Tubulin transfected 293T: (B) and K-562 (C) whole cell lysates.Application
Western blot analysis of β2B Tubulin expression in non-transfected 293T: (A), human β2B Tubulin transfected 293T: (B) and K-562 (C) whole cell lysates.Application
Western blot analysis of β2C Tubulin expression in non-transfected 293T: (A), mouse β2C Tubulin transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.Application
Western blot analysis of β5 Tubulin expression in non-transfected 293T: (A), human β5 Tubulin transfected 293T: (B) and BJAB (C) whole cell lysates.Application
Western blot analysis of β Tubulin expression in non-transfected 293T: (A), human β Tubulin transfected 293T: (B) and PC-12 (C) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human breast tissue showing cytoplasmic staining of glandular cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of β Tubulin expression in non-transfected 293T: (A), human β2C Tubulin transfected 293T: (B) and A-431 (C) whole cell lysates.Application
Western blot analysis of β Tubulin expression in non-transfected 293T: (A), human β2C Tubulin transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of β Tubulin expression in non-transfected 293T: (A), human β4 Tubulin transfected 293T: (B) and A-431 (C) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor showing cytoplasmic staining.Application
Western blot analysis of β Tubulin expression in A-431 whole cell lysate.| Product Name | β Tubulin Antibody (H-235) TRITC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 210-444 mapping at the C-terminus of β Tubulin of human origin |
| Modification Notes | Tubulin is a major cytoskeleton component that has five distinct forms, designated a, b, g, d and e tubulin. a and b tubulins form heterodimers which multimerize to form a microtubule filament. Multiple b Tubulin isoforms (b1, b2, b3, b4, b5, b6 and b8) have been characterized and are expressed in mammalian tissues. b1 and b4 are present throughout the cytosol, b2 is present in the nuclei and nucleoplasm, and b3 is a neuron-specific cytoskeletal protein. g Tubulin forms the gammasome, which is required for nucleating microtubule filaments at the centrosome. Both d Tubulin and e Tubulin are associated with the centrosome. d Tubulin is a homolog of the Chlamydomonas d Tubulin Uni3 and is found in association with the centrioles, whereas e Tubulin localizes to the pericentriolar material. e Tubulin exhibits a cell cycle-specific pattern of localization; first associating with only the older of the centrosomes in a newly duplicated pair, and later associating with both centrosomes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of β Tubulin of mouse, rat, human, zebrafish, C. elegans, Drosophila and Xenopus origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 210-444 mapping at the C-terminus of &beta Tubulin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 210-444 mapping at the C-terminus of β Tubulin of human origin |
| Entrez Gene | 7280 |
|---|
Application
Western blot analysis of β Tubulin expression in A-431 (A), BJAB (B), NIH/3T3 (C) and KNRK (D) whole cell lysates.
Application
Western blot analysis of β2A Tubulin expression in non-transfected 293T: (A), human β2A Tubulin transfected 293T: (B) and A-431 (C) whole cell lysates.
Application
Western blot analysis of β2C Tubulin expression in non-transfected 293T: (A), mouse β2C Tubulin transfected 293T: (B) and K-562 (C) whole cell lysates.
Application
Western blot analysis of β2B Tubulin expression in non-transfected 293T: (A), human β2B Tubulin transfected 293T: (B) and K-562 (C) whole cell lysates.
Application
Western blot analysis of β2C Tubulin expression in non-transfected 293T: (A), mouse β2C Tubulin transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.
Application
Western blot analysis of β5 Tubulin expression in non-transfected 293T: (A), human β5 Tubulin transfected 293T: (B) and BJAB (C) whole cell lysates.
Application
Western blot analysis of β Tubulin expression in non-transfected 293T: (A), human β Tubulin transfected 293T: (B) and PC-12 (C) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human breast tissue showing cytoplasmic staining of glandular cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of β Tubulin expression in non-transfected 293T: (A), human β2C Tubulin transfected 293T: (B) and A-431 (C) whole cell lysates.
Application
Western blot analysis of β Tubulin expression in non-transfected 293T: (A), human β2C Tubulin transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of β Tubulin expression in non-transfected 293T: (A), human β4 Tubulin transfected 293T: (B) and A-431 (C) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor showing cytoplasmic staining.
Application
Western blot analysis of β Tubulin expression in A-431 whole cell lysate.| Application Notes | recommended for detection of β Tubulin of mouse, rat, human, zebrafish, C. elegans, Drosophila and Xenopus origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
