Application
Western blot analysis of ERK 1 expression in non-transfected: (A) and human ERK 1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of ERK 1 expression in 293T (A) and A-431 (B) whole cell lysates.Application
Western blot analysis of ERK 1 expression in non-transfected: (A) and mouse ERK 1 transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic and nuclear localization using indirect FITC (A) staining and HeLa cells showing cytoplasmic localization using direct Alexa Fluor 488 (B) staining.Application
Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal rabbit IgG: .Application
Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal rabbit IgG: .Application
Intracellular FCM analysis of fixed and permeabilized A-431 cells. Black line histogram represents the isotype control, normal rabbit IgG: .Application
Immunofluorescence staining of a mixed population of NIH/3T3 cells showing nuclear localization of ERK 1 in four cells and cytoplasmic localization in the rest of the population.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded normal human breast tissue showing nuclear and cytoplasmic staining of ductal epithelia.Application
Antibodies tested include a phospho-ERK-specific antibody, p-ERK (E-4): (A,B) and a control antibody, ERK 1 (K-23): (C,D)Application
Antibodies tested include ERK 1 (K-23)-G: (A-C) and ERK 2 (C-14)-G: (D-F).| Product Name | ERK 1 Antibody (K-23) PE |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping within subdomain XI of ERK 1-encoded MAP kinase p44 of rat origin |
| Modification Notes | Mitogen-activated protein kinase (MAPK) signaling pathways involve two closely related MAP kinases, known as extracellular-signal-related kinase 1 (ERK 1, p44) and 2 (ERK 2, p42). Growth factors, steroid hormones, G protein-coupled receptor ligands and neurotransmitters can initiate MAPK signaling pathways. Activation of ERK 1 and ERK 2 requires phosphorylation by upstream kinases such as MAP kinasekinase (MEK), MEK kinase and Raf-1. ERK 1 and ERK 2 phosphorylation can occur at specific tyrosine and threonine sites mapping within consensus motifs that include the threonine-glutamate-tyrosine motif. ERK activation leads to dimerization with other ERKs and subsequent localization to the nucleus. Active ERK dimers phosphorylate serine and threonine residues on nuclear proteins and influence a host of responses that include proliferation, differentiation, transcription regulation and development. The human ERK 1 gene maps to chromosome 16p12-p11.2 and encodes a 379 amino acid protein that shares 83% sequence identity to ERK 2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCFCELISA |
| recommended for detection of ERK 1 p44 and, to a lesser extent, ERK 2 p42 of mouse, rat, human, avian, frog and zebrafish origin by WB, IP, IF, IHC(P), FCM and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within subdomain XI of ERK 1-encoded MAP kinase p44 of rat origin |
|---|---|
| Tissue Specificity | epitope mapping within subdomain XI of ERK 1-encoded MAP kinase p44 of rat origin |
| Entrez Gene | 5595 |
|---|
Application
Western blot analysis of ERK 1 expression in non-transfected: (A) and human ERK 1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of ERK 1 expression in 293T (A) and A-431 (B) whole cell lysates.
Application
Western blot analysis of ERK 1 expression in non-transfected: (A) and mouse ERK 1 transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic and nuclear localization using indirect FITC (A) staining and HeLa cells showing cytoplasmic localization using direct Alexa Fluor 488 (B) staining.
Application
Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Intracellular FCM analysis of fixed and permeabilized A-431 cells. Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Immunofluorescence staining of a mixed population of NIH/3T3 cells showing nuclear localization of ERK 1 in four cells and cytoplasmic localization in the rest of the population.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded normal human breast tissue showing nuclear and cytoplasmic staining of ductal epithelia.
Application
Antibodies tested include a phospho-ERK-specific antibody, p-ERK (E-4): (A,B) and a control antibody, ERK 1 (K-23): (C,D)
Application
Antibodies tested include ERK 1 (K-23)-G: (A-C) and ERK 2 (C-14)-G: (D-F).| Application Notes | recommended for detection of ERK 1 p44 and, to a lesser extent, ERK 2 p42 of mouse, rat, human, avian, frog and zebrafish origin by WB, IP, IF, IHC(P), FCM and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
