Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lung cancer tissue showing cytoplasmic staining of tumor cells.Application
Antibodies tested include p-NOS3 (Thr 495)-R: (A,B) and NOS3 (C-20): (C,D).Application
Western blot analysis of NOS3 expression in non-transfected: (A) and mouse NOS3 transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of methanol-fixed A549 cells showing cytoplasmic localization using indirect FITC (A) staining and HeLa cells using direct Alexa Fluor 488 (B) staining.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human ovary tumor (A) and immunofluorescence staining of methanol-fixed A549 cells (B) showing cytoplasmic localization.Application
Western blot analysis of NOS3 expression in rat brain extract.| Product Name | NOS3 Antibody (C-20) FITC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of NOS3 of human origin |
| Modification Notes | Nitric oxide (NO) has a broad range of biological activities and has been implicated in signaling pathways in phylogenetically diverse species. Nitric oxide synthases (NOSs), the enzymes responsible for synthesis of NO, contain an N-terminal oxygenase domain and a C-terminal reductase domain. NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells. The inducible form of NOS, NOS2 (also designated iNOS), is Ca2+-independent and is expressed in a broad range of cell types. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of NOS3 (ecNOS) of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of NOS3 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of NOS3 of human origin |
| Entrez Gene | 4846 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lung cancer tissue showing cytoplasmic staining of tumor cells.
Application
Antibodies tested include p-NOS3 (Thr 495)-R: (A,B) and NOS3 (C-20): (C,D).
Application
Western blot analysis of NOS3 expression in non-transfected: (A) and mouse NOS3 transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed A549 cells showing cytoplasmic localization using indirect FITC (A) staining and HeLa cells using direct Alexa Fluor 488 (B) staining.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human ovary tumor (A) and immunofluorescence staining of methanol-fixed A549 cells (B) showing cytoplasmic localization.
Application
Western blot analysis of NOS3 expression in rat brain extract.| Application Notes | recommended for detection of NOS3 (ecNOS) of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
