Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.Application
Western blot analysis of Flk-1 expression in non-transfected: (A) and mouse Flk-1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Flk-1 expression in mouse liver extract.| Product Name | Flk-1 Antibody (C-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of Flk-1 of mouse origin |
| Modification Notes | Three cell membrane receptor tyrosine kinases, Flt (also designated VEGF-R1) (1–3), Flk-1 (also designated VEGF-R2) (4–6) and Flt-4 (7), putatively involved in the growth of endothelial cells, are characterized by the presence of seven immunoglobulin-like sequences in their extracellular domain. These receptors exhibit high degrees of sequence relatedness to each other as well as lesser degrees of relatedness to the class III receptors including CSF-1/Fms, PDGR, SLFR/Kit and Flt-3/Flk-2. Two members of this receptor class, Flt-1 and Flk-1, have been shown to represent high affinity receptors for vascular endothelial growth factors (VEGFs) (3,5). On the basis of structural similarity to Flt and Flk-1, it has been speculated that Flt-4 might represent a third receptor for either VEGF or a VEGF-related ligand (7). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Flk-1 of mouse and rat origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of Flk-1 of mouse origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of Flk-1 of mouse origin |
| Entrez Gene | 16542 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.
Application
Western blot analysis of Flk-1 expression in non-transfected: (A) and mouse Flk-1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Flk-1 expression in mouse liver extract.| Application Notes | recommended for detection of Flk-1 of mouse and rat origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
