Application
Western blot analysis of ErbB-3 expression in T-47D whole cell lysate.Application
Western blot analysis of ErbB-3 expression in non-transfected: (A) and human ErbB-3 transfected: (B) 293T whole cell lysates.Application
Antibodies tested include ErbB-3 (G-4): (A-D) and ErbB-3 (C-17): (E-H).Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells transfected with ErbB-3 showing membrane localization (A). Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing membrane and cytoplasmic staining of epithelial cells (B).Application
Western blot analysis of ErbB-3 transfected NIH/3T3 cells.Application
Intracellular FCM analysis of fixed and permeabilized control (green line histogram) and ErbB-3 transfected (solid orange histogram) NIH/3T3 cells. Dotted pink histogram represents the isotype control, normal rabbit IgG: .| Product Name | ErbB-3 Antibody (C-17) AC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of ErbB-3 of human origin |
| Modification Notes | The EGF receptor family comprises several related receptor tyrosine kinases that are frequently overexpressed in a variety of carcinomas. Members of this receptor family include EGFR (HER1), Neu (ErbB-2, HER2), ErbB-3 (HER3) and ErbB-4 (HER4), which form either homodimers or heterodimers upon ligand binding. Full length ErbB-3 is overexpressed in human mammary tumors. The ErbB-3 gene also produces several alternative variants, including a secreted form which negatively regulates heregulin stimulated ErbB activation. ErbB-3 heterodimerizes with Neu and binds heregulin in order to activate phosphoinositide (PI) 3-kinase. The recruitment and activation of PI 3-kinase occurs via its interaction with phosphorylated YXXM motifs in the carboxy terminus of ErbB-3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCFCELISA |
| recommended for detection of ErbB-3 p160 of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of ErbB-3 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of ErbB-3 of human origin |
| Entrez Gene | 2065 |
|---|
Application
Western blot analysis of ErbB-3 expression in T-47D whole cell lysate.
Application
Western blot analysis of ErbB-3 expression in non-transfected: (A) and human ErbB-3 transfected: (B) 293T whole cell lysates.
Application
Antibodies tested include ErbB-3 (G-4): (A-D) and ErbB-3 (C-17): (E-H).
Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells transfected with ErbB-3 showing membrane localization (A). Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing membrane and cytoplasmic staining of epithelial cells (B).
Application
Western blot analysis of ErbB-3 transfected NIH/3T3 cells.
Application
Intracellular FCM analysis of fixed and permeabilized control (green line histogram) and ErbB-3 transfected (solid orange histogram) NIH/3T3 cells. Dotted pink histogram represents the isotype control, normal rabbit IgG: .| Application Notes | recommended for detection of ErbB-3 p160 of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
