Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human bronchus tissue showing nuclear and cytoplasmic staining of respiratory epithelial cells and glandular cells.Application
Western blot analysis of GATA-2 expression in non-transfected 293T: (A), human GATA-2 transfected 293T: (B) and P815 (C) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed P815 cells showing nuclear localization.Application
Western blot analysis of GATA-2 expression in MCP-5 whole cell lysate.| Product Name | GATA-2 Antibody (H-116) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 120-235 mapping within an internal region of GATA-2 of human origin |
| Modification Notes | Members of the GATA family share a conserved zinc finger DNA-binding domain and are capable of binding the WGATAR consensus sequence. GATA-1 is erythroid-specific and is responsible for the regulated transcription of erythroid genes. It is an essential component in the generation of the erythroid lineage. GATA-2 is expressed in embryonic brain and liver, HeLa and endothelial cells, as well as in erythroid cells. Studies with a modified GATA consensus sequence, AGATCTTA, have shown that GATA-2 and GATA-3 recognize this mutated consensus while GATA-1 has poor recognition of this sequence. This indicates broader regulatory capabilities of GATA-2 and GATA-3 than GATA-1. GATA-3 is highly expressed in T lymphocytes. GATA-4, GATA-5 and GATA-6 comprise a subfamily of transcription factors. Both GATA-4 and GATA-6 are found in heart, pancreas and ovary; lung and liver tissues exhibit GATA-6, but not GATA-4 expression. GATA-5 expression has been observed in differentiated heart and gut tissues and is present throughout the course of development in the heart. Although expression patterns of the various GATA transcription factors may overlap, it is not yet apparent how the GATA factors are able to discriminate in binding their appropriate target sites. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of GATA-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 120-235 mapping within an internal region of GATA-2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 120-235 mapping within an internal region of GATA-2 of human origin |
| Entrez Gene | 2624 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human bronchus tissue showing nuclear and cytoplasmic staining of respiratory epithelial cells and glandular cells.
Application
Western blot analysis of GATA-2 expression in non-transfected 293T: (A), human GATA-2 transfected 293T: (B) and P815 (C) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed P815 cells showing nuclear localization.
Application
Western blot analysis of GATA-2 expression in MCP-5 whole cell lysate.| Application Notes | recommended for detection of GATA-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
