Application
Western blot analysis of Fra-2 expression in non-transfected: (A) and human Fra-2 transfected: (B) whole cell lysates.Application
Western blot analysis of Fos B expression in non-transfected: (A) and human Fos B transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Fra-2 expression in non-transfected: (A) and human Fra-2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Fos B expression in non-transfected: (A) and human Fos B transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Fos gene family protein expression in untreated (A) and PMA-treated (B) Jurkat nuclear extracts.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human colon carcinoma tissue (A) and immunofluorescence staining of methanol-fixed A-431 cells (B) showing nuclear localization.Application
Intracellular FCM analysis of fixed and permeabilized MIA PaCa-2a cells. Black line histogram represents the isotype control, normal rabbit IgG: .WB
(A and B) Western blotting bands (a) and their statistical analyses (b) of c-Fos (A, n = 6) and pERK1/2 (B, n = 7).WB
A: Representative Western blotting bands (a) and the summary graph (b) showing c-Fos protein expression at day 5.| Product Name | c-Fos Antibody (K-25) AC |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Modification Notes | The c-Fos oncogene was initially detected in two independent murine osteosarcoma virus isolates and an avian nephroblastoma virus. The cellular homolog, c-Fos, encodes a nuclear phospho-protein that is rapidly and transiently induced by a variety of agents and functions as a transcriptional regulator for several genes. In contrast to c-Jun proteins, which form homo- and heterodimers which bind to specific DNA response elements, c-Fos proteins are only active as heterodimers with members of the Jun gene family. Functional homologs of c-Fos include the Fra-1, Fra-2 and Fos B genes. In addition, selected ATF/CREB family members can form leucine zipper dimers with Fos and Jun. Different dimers exhibit differential specificity and affinity for AP-1 and CRE sites. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ChIPELISAFCGSIFIHCIPWB |
| recommended for detection of c-Fos of mouse, rat, human and Xenopus origin by WB, IP, IF, IHC(P), FCM and ELISA also reactive with additional species, including equine, canine, bovine, porcine, avian and feline: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within an internal region of c-Fos of human origin |
|---|---|
| Tissue Specificity | epitope mapping within an internal region of c-Fos of human origin |
| Entrez Gene | 2353 |
|---|
Application
Western blot analysis of Fra-2 expression in non-transfected: (A) and human Fra-2 transfected: (B) whole cell lysates.
Application
Western blot analysis of Fos B expression in non-transfected: (A) and human Fos B transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Fra-2 expression in non-transfected: (A) and human Fra-2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Fos B expression in non-transfected: (A) and human Fos B transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Fos gene family protein expression in untreated (A) and PMA-treated (B) Jurkat nuclear extracts.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human colon carcinoma tissue (A) and immunofluorescence staining of methanol-fixed A-431 cells (B) showing nuclear localization.
Application
Intracellular FCM analysis of fixed and permeabilized MIA PaCa-2a cells. Black line histogram represents the isotype control, normal rabbit IgG: .
WB
(A and B) Western blotting bands (a) and their statistical analyses (b) of c-Fos (A, n = 6) and pERK1/2 (B, n = 7).
WB
A: Representative Western blotting bands (a) and the summary graph (b) showing c-Fos protein expression at day 5.| Application Notes | recommended for detection of c-Fos of mouse, rat, human and Xenopus origin by WB, IP, IF, IHC(P), FCM and ELISA also reactive with additional species, including equine, canine, bovine, porcine, avian and feline: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
