Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor (A) and immunofluorescence staining of methanol-fixed HeLa cells (B) showing cytoplasmic localization.Application
Western blot analysis of LAMP-1 expression in HeLa (A) and U-937 (B) whole cell lysates.| Product Name | LAMP-1 Antibody (H-228) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-228 of LAMP-1 of human origin |
| Modification Notes | Lysosome-associated membrane proteins (LAMP) are glycosylated type I membrane proteins that play a role in the biogenesis of the pigment melanin. LAMP-1 (also designated CD107a) and LAMP-2 (also designated CD107b) are involved in a variety of functions, including cellular adhesion, and are thought to participate in the process of tumor invasion and metastasis. Newly synthesized LAMP-1 and LAMP-2 proteins are sorted at the trans-Golgi network and are transported intracellularly via a pathway that is distinct from the Clathrin-coated vesicles used for the mannose-6 phosphate receptor. LAMP-1 is expressed on the surface of Thrombin-activated but not resting platelets, and it is thought to be involved in the adhesive, prothrombic properties of these cells. Both LAMP-1 and LAMP-2 are involved in maintaining lysosome acidity and protecting the lysosomal membranes from autodigestion, and their expression is increased in patients with lysosomal storage disorders. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of LAMP-1 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-228 of LAMP-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-228 of LAMP-1 of human origin |
| Entrez Gene | 3916 |
|---|
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor (A) and immunofluorescence staining of methanol-fixed HeLa cells (B) showing cytoplasmic localization.
Application
Western blot analysis of LAMP-1 expression in HeLa (A) and U-937 (B) whole cell lysates.| Application Notes | recommended for detection of LAMP-1 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
