Application
Western blot analysis of PCAF expression in K-562 (A) and BJAB (B) whole cell lysates.Application
Antibodies tested include CBP (A-22): , p300 (N-15): , PCAF (H-369): , and Brg-1 (H-88): . CD3ε and Oct-2 promoter regions were employed as positive and negative controls, respectively. DNA was isolated from Rag-/- thymocytes or Rag-/- thymocytes expressing TCRβ. Data kindly provided by J. Imbert and reproduced from Yeh, J-H., et al., Nucleic Acids Research, 2002, 30: 1944-1951, with permission from Oxford University Press.Application
Western blot analysis of His-tagged human recombinant PCAF.Application
Antibodies tested include NCoA-3 (F-2): , NCoA-3 (M-397): , NCoA-3 (N-17): , NCoA-3 (C-20): , SRC-1 (M-341): , SRC-1 (C-20): , SRC-1 (M-20): , GRIP1 (M-343): , PCAF (C-16): and PCAF (H-369): . Data kindly provided by M.G. Rosenfeld and reproduced with permission from Baek et al., Cell 2002, 110: 55-67.| Product Name | PCAF Antibody (H-369) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 464-832 mapping at the C-terminus of p300/CBP-associated factor (PCAF) of human origin |
| Conjugation | Agarose |
| Modification | Acetylated |
| Modification Notes | In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (for p300/CBP-associated factor), p300/CBP and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1) and HDAC2 (also designated mammalian RPD3), both of which are related to the yeast transcriptional regulator Rpd3p, have been identified as histone deacetylases. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ChIPELISAIFIPWB |
| WB:1:100~1:400 IP:1:20~1:100 IF:1:20~1:100 |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | equine, canine, bovine, porcine and avian |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 464-832 mapping at the C-terminus of p300/CBP-associated factor (PCAF) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 464-832 mapping at the C-terminus of p300/CBP-associated factor (PCAF) of human origin |
| Entrez Gene | 8850 |
|---|
Application
Western blot analysis of PCAF expression in K-562 (A) and BJAB (B) whole cell lysates.
Application
Antibodies tested include CBP (A-22): , p300 (N-15): , PCAF (H-369): , and Brg-1 (H-88): . CD3ε and Oct-2 promoter regions were employed as positive and negative controls, respectively. DNA was isolated from Rag-/- thymocytes or Rag-/- thymocytes expressing TCRβ. Data kindly provided by J. Imbert and reproduced from Yeh, J-H., et al., Nucleic Acids Research, 2002, 30: 1944-1951, with permission from Oxford University Press.
Application
Western blot analysis of His-tagged human recombinant PCAF.
Application
Antibodies tested include NCoA-3 (F-2): , NCoA-3 (M-397): , NCoA-3 (N-17): , NCoA-3 (C-20): , SRC-1 (M-341): , SRC-1 (C-20): , SRC-1 (M-20): , GRIP1 (M-343): , PCAF (C-16): and PCAF (H-369): . Data kindly provided by M.G. Rosenfeld and reproduced with permission from Baek et al., Cell 2002, 110: 55-67.| Application Notes | WB:1:100~1:400 IP:1:20~1:100 IF:1:20~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
