Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic and membrane staining of cells in glomeruli and tubuli. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney carcinoma showing membrane and cytoplasmic localization.Application
Western blot analysis of LAMP-2 expression in U-937 whole cell lysate.| Product Name | LAMP-2 Antibody (H-207) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-207 mapping at the N-terminus of LAMP-2 (lysosome-associated membrane glycoprotein-2) of human origin |
| Modification Notes | Lysosome-associated membrane proteins (LAMP) are glycosylated type I membrane proteins that play a role in the biogenesis of the pigment melanin (1). LAMP-1 (also designated CD107A) and LAMP-2 (also designated CD107B) are involved in a variety of functions, including cellular adhesion, and are thought to participate in the process of tumor invasion and metastasis (2,3). Newly synthesized LAMP-1 and LAMP-2 proteins are sorted at the trans Golgi network and are transported intracellularly via a pathway that is distinct from the clathrin-coated vesicles used for the mannose-6 phosphate receptor (4). LAMP-1 is expressed on the surface of thrombin-activated but not resting platelets, and it is thought to be involved in the adhesive, prothrombic properties of these cells (5). Both LAMP-1 and LAMP-2 are involved in maintaining lysosome acidity and protecting the lysosomal membranes from autodigestion, and their expression is increased in patients with lysosomal storage disorders (6). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of LAMP-2 human and, to a lesser extent, mouse and rat by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-207 mapping at the N-terminus of LAMP-2 (lysosome-associated membrane glycoprotein-2) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-207 mapping at the N-terminus of LAMP-2 (lysosome-associated membrane glycoprotein-2) of human origin |
| Entrez Gene | 3920 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic and membrane staining of cells in glomeruli and tubuli. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney carcinoma showing membrane and cytoplasmic localization.
Application
Western blot analysis of LAMP-2 expression in U-937 whole cell lysate.| Application Notes | recommended for detection of LAMP-2 human and, to a lesser extent, mouse and rat by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
