Application
Western blot analysis of Dynactin 1 expression in Ramos whole cell lysate.Application
Western blot analysis of Dynactin 1 expression in non-transfected 293: (A), human Dynactin 1 transfected 29T: (B) and HeLa (C) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human stomach tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.Application
Western blot analysis of Dynactin 1 expression in mouse (A) and rat (B) brain tissue extract.| Product Name | Dynactin 1 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 964-1263 of Dynactin 1of human origin |
| Modification Notes | Dynactin, a multisubunit complex, is a cytoplasmic Dynein-interacting protein that functions as the ”receptor” for the Dynein microtubule motor. Dynactin/ Dynein binding may be required for most, if not all, cytoplasmic Dynein-driven activities and is thought to contribute to the functional diversity of Dynein. Enriched in neurons, Dynactin also binds to microtubules and has been shown to function in diverse processes, including organelle transport, formation of the mitotic spindle and cytokinesis. Dynactin subunits include p22, p50, p62, p150 (also designated Glued) and ARP-1. The p135 splice variant is neuron specific and, unlike p150, does not bind microtubules. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of p150 and p135 splice variants of Dynactin 1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 964-1263 of Dynactin 1of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 964-1263 of Dynactin 1of human origin |
| Entrez Gene | 1639 |
|---|
Application
Western blot analysis of Dynactin 1 expression in Ramos whole cell lysate.
Application
Western blot analysis of Dynactin 1 expression in non-transfected 293: (A), human Dynactin 1 transfected 29T: (B) and HeLa (C) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human stomach tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.
Application
Western blot analysis of Dynactin 1 expression in mouse (A) and rat (B) brain tissue extract.| Application Notes | recommended for detection of p150 and p135 splice variants of Dynactin 1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
