Application
Western blot analysis of p107 expression in non-transfected 293T: (A), truncated human p107 transfected 293T: (B) and SK-BR-3 (C) whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human colon carcinoma tissue showing nuclear localization.Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing nuclear localization.Application
Western blot analysis of p107 expression in SK-BR-3 (A), C32 (B), NIH/3T3 (C), MM-142 (D), KNRK (E) and 3611-RF (F) whole cell lysates.Application
Western blot analysis of p107 expression in C32 (A), MM-142 (B) and KNRK (C) whole cell lysates.| Product Name | p107 Antibody (C-18) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of p107 of human origin |
| Modification Notes | The human retinoblastoma gene product plays an important role in the negative regulation of cell proliferation. Functional inactivation of Rb can be mediated either through mutation or as a consequence of interaction with DNA tumor virus encoded proteins. pRb and the structurally related p107 form complexes with E2F, a transcription factor originally identified through its role in transcriptional activation of the adenovirus E2 promoter. Moreover, pRB and p107 share a high degree of structural homology in the adenovirus E1A binding domain (i.e. “pocket region”) that is believed to play a primary role in the function of these proteins. A protein designated p130 shows a high degree of identity with pRb and p107 and also possesses a pocket region. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of p107 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of p107 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of p107 of human origin |
| Entrez Gene | 5933 |
|---|
Application
Western blot analysis of p107 expression in non-transfected 293T: (A), truncated human p107 transfected 293T: (B) and SK-BR-3 (C) whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human colon carcinoma tissue showing nuclear localization.
Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing nuclear localization.
Application
Western blot analysis of p107 expression in SK-BR-3 (A), C32 (B), NIH/3T3 (C), MM-142 (D), KNRK (E) and 3611-RF (F) whole cell lysates.
Application
Western blot analysis of p107 expression in C32 (A), MM-142 (B) and KNRK (C) whole cell lysates.| Application Notes | recommended for detection of p107 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
