Application
Western blot analysis of spectrin α I expression in HEL 92.1.7 (A) and K-562 (B) whole cell lysates and rat heart tissue extract (C).| Product Name | spectrin α I Antibody (H-110) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 541-650 of spectrin α I of human origin |
| Modification Notes | Spectrin, an Actin binding protein that is a major component of the cytoskeletal superstructure of the erythrocyte plasma membrane, is essential in determining the properties of the membrane including its shape and deformability. Spectrins function as membrane organizers and stabilizers, composed of non-homologous a and b chains, which aggregate side-to-side in an antiparallel fashion to form dimers, tetramers and higher polymers. Spectrin a I and spectrin b I are present in erythrocytes, whereas spectrin a II (also designated fodrin a) and spectrin b II (also designated fodrin b) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function. Defects of spectrin a I are present in the erythrocytes of many patients with abnormalities of red blood cell shape including hereditary spherocytosis and elliptocytosis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of spectrin α I of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 541-650 of spectrin &alpha I of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 541-650 of spectrin α I of human origin |
| Entrez Gene | 6708 |
|---|
Application
Western blot analysis of spectrin α I expression in HEL 92.1.7 (A) and K-562 (B) whole cell lysates and rat heart tissue extract (C).| Application Notes | recommended for detection of spectrin α I of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
