Application
Western blot analysis of Caldesmon expression in non-transfected: (A) and human Caldesmon transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Caldesmon expression in non-transfected 293T: (A), truncated mouse Caldesmon transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoskeletal staining.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and membrane localization.Application
Western blot analysis of L-Caldesmon and H-Caldesmon expression in BC3H1 whole cell lysate.| Product Name | Caldesmon Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 494-793 of H-Caldesmon of human origin |
| Modification Notes | Caldesmon, Filamin 1, Nebulin and Villin are differentially expressed and regulated Actin binding proteins. Both muscular and non-muscular forms of Caldesmon have been identified and each has been shown to bind to Actin as well as to calmodulin and myosin. Alternative splicing of the gene encoding Caldesmon results in five isoforms. Muscular Caldesmon (isoform 1), also designated high molecular weight Caldesmon or H-Caldesmon (H-CAD), is expressed predominantly on thin filaments in smooth muscle. Non-muscular Caldesmon (isoforms 2-5), also designated low molecular weight Caldesmon or L-Caldesmon (L-CAD), is widely expressed in non-muscle tissues and cells. Filamin 1, which is ubiquitously expressed and exists as a homodimer, functions to crosslink Actin to filaments. Nebulin is a large filamentous protein specific to muscle tissue that may function as a ruler for filament length. Several isoforms of Nebulin are produced by alternative exon usage. Villin is Ca2+-regulated and is the major structural component of the brush border of absorptive cells. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of H-Caldesmon and L-Caldesmon I and II of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 494-793 of H-Caldesmon of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 494-793 of H-Caldesmon of human origin |
| Entrez Gene | 800 |
|---|
Application
Western blot analysis of Caldesmon expression in non-transfected: (A) and human Caldesmon transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Caldesmon expression in non-transfected 293T: (A), truncated mouse Caldesmon transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoskeletal staining.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and membrane localization.
Application
Western blot analysis of L-Caldesmon and H-Caldesmon expression in BC3H1 whole cell lysate.| Application Notes | recommended for detection of H-Caldesmon and L-Caldesmon I and II of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
