Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic localization.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of Syk phosphorylation expression in 3 minute H2O2 treated (A) and 15 minute H2O2 treated (B) JK cell extracts.| Product Name | p-Syk Antibody (Tyr 323) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Tyr 323 phosphorylated Syk of human origin by WB, IP, IF and IHC(P) |
| Modification | Phosphoralated |
| Modification Notes | Syk (spleen tyrosine kinase) is a 635 amino acid protein that contains one protein kinase domain and two SH2 domains. One of several members of the protein kinase superfamily, Syk functions as a positive effector of B cell antigen receptor (CD79)-stimulated responses, coupling CD79 with the movement of one calcium ion through one of two phospho-regulated pathways. Specifically, calcium ions travel through either a phosphoinositide 3-kinase (PI 3-kinase)-dependent pathway when Syk is not phosphorylated, or through a phospholipase C (PLC) ©-dependent pathway when human Syk is phohsphorylated on Tyr 348 and Tyr 352. Via its ability to influence CD79 activity and to control the movement of calicum through the cell, Syk plays an important role in a variety of cellular responses, including differentiation, phagocytosis, proliferation and B cell development. Syk is phosphorylated by Lyn on Tyr 323, creating a binding site for Cbl. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Tyr 323 phosphorylated Syk of human origin by WB IP IF and IHC(P) |
|---|---|
| Tissue Specificity | recommended for detection of Tyr 323 phosphorylated Syk of human origin by WB, IP, IF and IHC(P) |
| Entrez Gene | 6850 |
|---|
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic localization.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of Syk phosphorylation expression in 3 minute H2O2 treated (A) and 15 minute H2O2 treated (B) JK cell extracts.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
