Application
Western blot analysis of Stat5a expression in non-transfected: (A) and human Stat5a transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Stat5a expression in non-transfected: (A) and human Stat5a transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Stat5a expression in non-transfected: (A) and human Stat5a transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Stat5a expression in K-562 whole cell lysate.Application
Indirect, intracellular FCM analysis of fixed and permeabilized K-562 cells stained with Stat5a (L-20), followed by PE-conjugated goat anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .Application
Indirect, intracellular FCM analysis of fixed and permeabilized K-562 cells stained with Stat5a (L-20), followed by FITC-conjugated goat anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .Application
Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear staining.Application
Western blot analysis of Stat5a expression in K-562 nuclear extract.| Product Name | Stat5a Antibody (L-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of Stat5a of mouse origin |
| Modification Notes | Signal transducer and activator of transcription 5A (Stat5a) and Stat5b, which share 96% homology, undergo receptor tyrosine kinase or G protein-coupled receptor-dependent phosphorylation in response to cytokines or growth factors, and then form homo- or heterodimers that translocate to the nucleus, where they initiate transcription. Activation of Stat5a via IL-2, IL-3, IL-7/ GM-CSF, erythropoietin, thrombopoietin and growth hormones influences proliferation, differentiation and apoptosis in lymphohematopoietic cells. Phos-phorylation of Stat5a at Ser127/Ser128 and Ser779 are contigent on ErbB-4-mediated activation of Stat5a. Activation of Stat5b via IL-2, IL-4, CSF-1 and growth hormones influences TCR signaling, apoptosis, adult mammary gland development and sexual dimorphism of liver gene expression. Stat5b is the major liver-expressed Stat5 form that has been shown to fuse with the retin-oic acid receptor a gene in acute promyelocytic leukemias (APLL). Stat5a/b null mice have severely impaired lymphoid development and differentiation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFFCELISAGSChIP |
| recommended for detection of Stat5a of mouse, rat and human origin by WB, IP, IF, ELISA and indirect FCM using PE (sc-3739)- or FITC (sc-2012)-conjugated goat anti-rabbit IgG non cross-reactive with Stat5b also reactive with additional species, including equine and canine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of Stat5a of mouse origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of Stat5a of mouse origin |
| Entrez Gene | 6776 |
|---|
Application
Western blot analysis of Stat5a expression in non-transfected: (A) and human Stat5a transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Stat5a expression in non-transfected: (A) and human Stat5a transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Stat5a expression in non-transfected: (A) and human Stat5a transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Stat5a expression in K-562 whole cell lysate.
Application
Indirect, intracellular FCM analysis of fixed and permeabilized K-562 cells stained with Stat5a (L-20), followed by PE-conjugated goat anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Indirect, intracellular FCM analysis of fixed and permeabilized K-562 cells stained with Stat5a (L-20), followed by FITC-conjugated goat anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear staining.
Application
Western blot analysis of Stat5a expression in K-562 nuclear extract.| Application Notes | recommended for detection of Stat5a of mouse, rat and human origin by WB, IP, IF, ELISA and indirect FCM using PE (sc-3739)- or FITC (sc-2012)-conjugated goat anti-rabbit IgG non cross-reactive with Stat5b also reactive with additional species, including equine and canine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
