Application
Western blot analysis of human recombinant NFκB p50 (A) and GST (B) fusion proteins.| Product Name | GST Antibody (Z-5) Alexa Fluor® 405 |
|---|---|
| Antibody Type | Tags Antibodies |
| Antigen Alias | recommended for detection of glutathione-S-transferase (GST) of Schistosoma japonicum origin and GST fusion proteins |
| Modification Notes | Plasmid vectors for the expression of coding regions of eukaryotic genes in E. coli are in common usage; such expression vectors often encode hybrid fusion proteins containing part prokaryotic and part eukaryotic specified proteins. For instance, the pGEX.3X expression vector developed by Smith and Johnson allows for synthesis of fusion proteins between glutathionine-S-transferase (GST) and proteins encoded by inserted cDNA sequences. Antibodies derived from these GST fusion proteins are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in E. coli. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFFCELISA |
| WB, IP, IF, FCM and ELISA of recombinant GST fusion proteins expressed in E. coli designed to be used with GST expression vectors such as pGEX.3X and pGEX.2T: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of glutathione-S-transferase (GST) of Schistosoma japonicum origin and GST fusion proteins |
|---|---|
| Tissue Specificity | recommended for detection of glutathione-S-transferase (GST) of Schistosoma japonicum origin and GST fusion proteins |
Application
Western blot analysis of human recombinant NFκB p50 (A) and GST (B) fusion proteins.| Application Notes | WB, IP, IF, FCM and ELISA of recombinant GST fusion proteins expressed in E. coli designed to be used with GST expression vectors such as pGEX.3X and pGEX.2T: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
