Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human vulva skin tissue showing membrane and cytoplasmic staining of epidermal cells.Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing membrane localization.Application
Western blot analysis of γ-catenin expression in non-transfected 293T: (A), human γ-catenin transfected 293T: (B) and A-431 (C) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane staining.Application
Western blot analysis of γ-catenin expression in A-431 (A) and HeLa (B) whole cell lysates.| Product Name | γ-catenin Antibody (H-80) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 30-109 mapping near the N-terminus of γ-catenin of human origin |
| Modification Notes | The catenins, å, ∫ and ©, are proteins which bind to the highly conserved, intracellular cytoplasmic tail of E-cadherin. Together, the catenin/cadherin complexes play an important role mediating cellular adhesion. å-catenin was initially described as an E-cadherin associated protein, and since has been shown to associate with other members of the cadherin family, such as N-cadherin and P-cadherin. ∫-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. ∫-catenin has also been found in complexes with the tumor suppressor protein APC. ©-catenin, also known as plakoglobin, binds with å-catenin and N-cadherin. It has been shown that the transmembrane phosphatase PTPµ associates with catenin/cadherin complexes and may regulate complex signaling. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of γ-catenin of mouse, rat and human origin by WB, IP, IF, IHC(P)and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 30-109 mapping near the N-terminus of &gamma -catenin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 30-109 mapping near the N-terminus of γ-catenin of human origin |
| Entrez Gene | 3728 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human vulva skin tissue showing membrane and cytoplasmic staining of epidermal cells.
Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing membrane localization.
Application
Western blot analysis of γ-catenin expression in non-transfected 293T: (A), human γ-catenin transfected 293T: (B) and A-431 (C) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane staining.
Application
Western blot analysis of γ-catenin expression in A-431 (A) and HeLa (B) whole cell lysates.| Application Notes | recommended for detection of γ-catenin of mouse, rat and human origin by WB, IP, IF, IHC(P)and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
