Application
Western blot analysis of NKHC1 expression in non-transfected 293T: (A), human NKHC1 transfected 293T: (B) and IMR-32 (C) whole cell lysates.Application
Western blot analysis of NKHC1 expression in C6 whole cell lysate.Application
Western blot analysis of NKHC1 expression in non-transfected: (A) and human NKHC1 transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebral cortex tissue showing nuclear and cytoplasmic staining of neuronal cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of NKHC1 expression in C6 (A) and IMR-32 (B) whole cell lysates.| Product Name | NKHC1 Antibody (H-75) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 506-580 of NKHC1 of human origin |
| Modification Notes | NKHC1 (neuronal kinesin heavy chain 1), also known as KIF5A; kinesin family member 5A, NKHC; kinesin heavy chain, neuron-specific, SPG10 and spastic paraplegia 10, is a neuronal-specific component of a multi-subunit “molecular motor” complex that mediates intracellular organelle transport. Mutations in the gene encoding NKHC1 cause autosomal dominant spastic paraplegia 10. NKHC1 has a pan-neuronal distribution in the nervous system. Rat tissue extracts by immunoblot of NKHC1 can produce a doublet only in brain and sciatic nerve tissue. NKHC1 is distributed throughout the central nervous system and is enriched in subsets of neurons. Within cultured hippocampal neurons, NKHC1 is concentrated in the perinuclear region of the cell body. Kinesin superfamily proteins like NKHC1 are the molecular motors conveying cargos along microtubules. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of neuronal kinesin heavy chain 1 (NKHC1) of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 506-580 of NKHC1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 506-580 of NKHC1 of human origin |
| Entrez Gene | 3798 |
|---|
Application
Western blot analysis of NKHC1 expression in non-transfected 293T: (A), human NKHC1 transfected 293T: (B) and IMR-32 (C) whole cell lysates.
Application
Western blot analysis of NKHC1 expression in C6 whole cell lysate.
Application
Western blot analysis of NKHC1 expression in non-transfected: (A) and human NKHC1 transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebral cortex tissue showing nuclear and cytoplasmic staining of neuronal cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of NKHC1 expression in C6 (A) and IMR-32 (B) whole cell lysates.| Application Notes | recommended for detection of neuronal kinesin heavy chain 1 (NKHC1) of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
