Application
Immunofluorescence staining of normal mouse eye frozen section showing basement membrane staining.Application
Immunofluorescence staining of methanol-fixed, control HeLa (A) and Laminin β-1 siRNA silenced HeLa (B) cells showing diminished cytoplasmic staining in the siRNA silenced cells. Cells probed with Laminin β-1 (H-300): .Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Immunofluorescence staining of methanol-fixed A-431 cells showing cytoplasmic staining.Application
Western blot analysis of Laminin β-1 expression in A-431 whole cell lysate.| Product Name | Laminin β-1 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1487-1786 mapping at the C-terminus of Laminin β-1 of human origin |
| Modification Notes | Laminins are essential and abundant structural non-collagenous glyco-proteins localizing to basement membranes. Basement membranes (cell-associated extracellular matrices (ECMs)) are polymers of laminins with stabilizing type IV collagen networks, nidogen, and several proteoglycans. Basement membranes are found under epithelial layers, around the endothelium of blood vessels, and surrounding muscle, peripheral nerve, and fat cells. Formation of basement membranes influences cell proliferation, phenotype, migration, gene expression, and tissue architecture. Each laminin is a heterotrimer of a, b, and g chain subunits that undergoes cell-secretion and incorporation into the ECM. Laminins can self-assemble, bind to other matrix macromolecules, and have unique and shared cell interactions mediated by integrins, dystroglycan, and cognate laminin receptors. The human Laminin b-1 gene maps to chromosome 7q22 and is ubiquitously expressed in tissues that produce basement membranes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Laminin β-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1487-1786 mapping at the C-terminus of Laminin &beta -1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1487-1786 mapping at the C-terminus of Laminin β-1 of human origin |
| Entrez Gene | 3912 |
|---|
Application
Immunofluorescence staining of normal mouse eye frozen section showing basement membrane staining.
Application
Immunofluorescence staining of methanol-fixed, control HeLa (A) and Laminin β-1 siRNA silenced HeLa (B) cells showing diminished cytoplasmic staining in the siRNA silenced cells. Cells probed with Laminin β-1 (H-300): .
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Immunofluorescence staining of methanol-fixed A-431 cells showing cytoplasmic staining.
Application
Western blot analysis of Laminin β-1 expression in A-431 whole cell lysate.| Application Notes | recommended for detection of Laminin β-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
