Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing membrane staining of glomerular cells and cytoplasmic staining of cells in tubules.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human colo-rectal cancer tissue showing cytoplasmic staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of neogenin expression in HeLa (A), Ca Ski (B), T84 (C) and A549 (D) whole cell lysates.| Product Name | neogenin Antibody (H-175) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1171-1345 of neogenin of human origin |
| Modification Notes | Neogenin (NGN) was first identified in chicken as a highly regulated protein in the developing nervous system and gastrointestinal tract (1). The human homolog is roughly 50% identical to the protein DCC (deleted in colorectal cancer), a candidate tumor suppressor that is also involved in neural development (2-4). DCC and neogenin may play complementary roles in the generation of the fully functional central nervous system (5). Neogenin is expressed in most normal tissues; in contrast to DCC, it is also detected at normal levels in cancer tissues (2,6). Neogenin is a member of the N-CAM family of cell adhesion molecules and is expressed on the surfaces of growing nerve cells as well as in a number of other developing embryonic tissues (6,7). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of neogenin of mouse, rat, human and zebrafish origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1171-1345 of neogenin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1171-1345 of neogenin of human origin |
| Entrez Gene | 4756 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing membrane staining of glomerular cells and cytoplasmic staining of cells in tubules.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human colo-rectal cancer tissue showing cytoplasmic staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of neogenin expression in HeLa (A), Ca Ski (B), T84 (C) and A549 (D) whole cell lysates.| Application Notes | recommended for detection of neogenin of mouse, rat, human and zebrafish origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
