Application
Intracellular FCM analysis of fixed and permeabilized A-431 cells. Black line histogram represents the isotype control, normal rabbit IgG: .Application
Western blot analysis of JNK2 expression in non-transfected 293T: (A), human JNK2 transfected 293T: (B) and K-562 (C) whole cell lysates.Application
Western blot analysis of JNK2 expression in non-transfected: (A) and human JNK2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of JNK2 expression in non-transfected: (A) and human JNK2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of JNK2 expression in non-transfected 293T: (A), mouse JNK2 transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.Application
Western blot analysis of JNK2 expression in non-transfected: (A) and mouse JNK2 transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.Application
Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal rabbit IgG: .Application
Intracellular FCM analysis of fixed and permeabilized A431 cells. Black line histogram represents the isotype control, normal rabbit IgG: .Application
Western blot analysis of JNK2 expression in control (A), JNK2-transfected (B) and UV-irradiated (C) COS whole cell lysates.| Product Name | JNK2 Antibody (N-18) PE |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the N-terminus of JNK2 of human origin |
| Modification Notes | c-Jun N-terminal kinases (JNKs) phosphorylate and augment transcriptional activity of c-Jun. JNKs originate from three genes that yield 10 isoforms through alternative mRNA splicing, including JNK1å1, JNK1∫1, JNK2å1, JNK2∫1, and JNK3å1, which represent the p46 isoforms, and JNK1å2, JNK1∫2, JNK2å2, JNK2∫2, and JNK3∫2, which represent the p54 isoforms.JNKs coordinate cell responses to stress and influence regulation of cell growth and transformation. The human JNK1 (PRKM8, SAPK1, MAPK8) gene maps to chromosome 10q11.22 and shares 83% amino acid identity with JNK2. JNK1 is necessary for normal activation and differentiation of CD4 helper T (TH) cells into TH1 and TH2 effector cells. Capsaicin activates JNK1 and p38 in ras-transformed human breast epithelial cells. Nitrogen oxides (NOx) upregulate JNK1 in addition to c-Fos, c-Jun, and other signaling kinases, including MEKK1 and p38. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFFCELISA |
| recommended for detection of JNK2 and, to a lesser extent, JNK1 and JNK3 of mouse, rat, human, and, to a lesser extent, monkey origin by WB, IP, IF, FCM and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the N-terminus of JNK2 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the N-terminus of JNK2 of human origin |
| Entrez Gene | 5601 |
|---|
Application
Intracellular FCM analysis of fixed and permeabilized A-431 cells. Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Western blot analysis of JNK2 expression in non-transfected 293T: (A), human JNK2 transfected 293T: (B) and K-562 (C) whole cell lysates.
Application
Western blot analysis of JNK2 expression in non-transfected: (A) and human JNK2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of JNK2 expression in non-transfected: (A) and human JNK2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of JNK2 expression in non-transfected 293T: (A), mouse JNK2 transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.
Application
Western blot analysis of JNK2 expression in non-transfected: (A) and mouse JNK2 transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.
Application
Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Intracellular FCM analysis of fixed and permeabilized A431 cells. Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Western blot analysis of JNK2 expression in control (A), JNK2-transfected (B) and UV-irradiated (C) COS whole cell lysates.| Application Notes | recommended for detection of JNK2 and, to a lesser extent, JNK1 and JNK3 of mouse, rat, human, and, to a lesser extent, monkey origin by WB, IP, IF, FCM and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
