Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing nuclear and cytoplasmic staining of cells in seminiferous ducts.Application
Antibodies tested include p-Rho A (Ser 188): (A,B) and Rho A (119): (C,D).Application
Western blot analysis of Rho A expression in non-transfected: (A) and human Rho A transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Rho A expression in non-transfected: (A) and human Rho A transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Rho A expression in Jurkat whole cell lysate.Application
Intracellular FCM analysis of fixed and permeabilized KNRK cells. Black line histogram represents the isotype control, normal rabbit IgG: .Application
Western blot analysis of Rho A expression in KNRK (A), PC-12 (B), HL-60 (C) and MCF7 (D) whole cell lysates.Application
Western blot analysis of Rho A expression in non-transfected control (A) and Rho A siRNA (B) transfected HeLa cells. Blot probed with Rho A (119): . GAPDH (V-18): used as specificity and loading control.Application
Cytoplasmic immunofluorescence staining of methanol/acetone-fixed rat embryo fibroblasts using fluorescein-labeled goat anti-rabbit IgG secondary antibody.Application
Antibodies tested include Rho A (119): (A) and Rho A (26C4): (B,C).| Product Name | Rho A Antibody (119) HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping to an internal region of Rho A of human origin |
| Modification Notes | The Ras p21 family of guanine nucleotide proteins has been widely studied in view of its apparent role in signal transduction pathways and high frequency of mutations in human malignancies. It is now clear, however, that the Ras proteins (H-, K- and N-Ras p21) are members of a much larger superfamily of related proteins. Six members of this family, Rap 1A, Rap 1B, Rap 2, R-Ras, Ral A and Ral B, exhibit approximately 50% amino acid homology to Ras. The six mammalian Rho proteins (Rho A, B, C, G, 7 and 8) are approximately 30% homologous to Ras and are expressed in a wide range of cell types. Both Ras p21 and Rho p21, as well as other members of the Ras superfamily, contain a carboxy-terminal CAAX sequence (C, cysteine; A, aliphatic amino acid; X, any amino acid) which in the case of Ras has been shown to be essential for correct localization and function. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCFCELISAChIP |
| recommended for detection of Rho A p21 and Rho C and, to a lesser extent, Rho B of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping to an internal region of Rho A of human origin |
|---|---|
| Tissue Specificity | epitope mapping to an internal region of Rho A of human origin |
| Entrez Gene | 387 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing nuclear and cytoplasmic staining of cells in seminiferous ducts.
Application
Antibodies tested include p-Rho A (Ser 188): (A,B) and Rho A (119): (C,D).
Application
Western blot analysis of Rho A expression in non-transfected: (A) and human Rho A transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Rho A expression in non-transfected: (A) and human Rho A transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Rho A expression in Jurkat whole cell lysate.
Application
Intracellular FCM analysis of fixed and permeabilized KNRK cells. Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Western blot analysis of Rho A expression in KNRK (A), PC-12 (B), HL-60 (C) and MCF7 (D) whole cell lysates.
Application
Western blot analysis of Rho A expression in non-transfected control (A) and Rho A siRNA (B) transfected HeLa cells. Blot probed with Rho A (119): . GAPDH (V-18): used as specificity and loading control.
Application
Cytoplasmic immunofluorescence staining of methanol/acetone-fixed rat embryo fibroblasts using fluorescein-labeled goat anti-rabbit IgG secondary antibody.
Application
Antibodies tested include Rho A (119): (A) and Rho A (26C4): (B,C).| Application Notes | recommended for detection of Rho A p21 and Rho C and, to a lesser extent, Rho B of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
