Application
Antibodies tested include c-Rel (N): (A) and c-Rel (N-466): (B).Application
Immunofluorescence staining of methanol-fixed KNRK cells showing cytoplasmic localization.| Product Name | c-Rel Antibody (N-466) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to 300 amino acids mapping within the N-terminus of c-Rel p75 of human origin |
| Modification Notes | c-Rel is the cellular cognate of v-Rel, the avian reticuloendotheliosis virus strain T transforming gene. v-Rel encodes a phosphoprotein that is located in the cytoplasm of transformed spleen cells and in the nucleus of non-transformed fibroblasts, in contrast to the c-Rel protein, which is cytoplasmic. c-Rel has been shown to represent a constituent of the ?B site binding transcription factor NF?B, which plays a crucial role in the expression of immunoglobulin ˚ light chain gene. In contrast to c-Rel, v-Rel is truncated in its C-terminal transactivation domain and does not appear to function as a transcriptional transactivator. It has thus been postulated that v-Rel may interfere with the normal transcription of NF?B regulated genes and thus cause transformation by a mechanism analogous to v-ErbA, which binds to the thyroid hormone-responsive region in certain erythroid genes needed for differentiation, but cannot be activated by thyroid hormone. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of c-Rel p75 and NFκB p65 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to 300 amino acids mapping within the N-terminus of c-Rel p75 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to 300 amino acids mapping within the N-terminus of c-Rel p75 of human origin |
| Entrez Gene | 5966 |
|---|
Application
Antibodies tested include c-Rel (N): (A) and c-Rel (N-466): (B).
Application
Immunofluorescence staining of methanol-fixed KNRK cells showing cytoplasmic localization.| Application Notes | recommended for detection of c-Rel p75 and NFκB p65 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
