Application
Western blot analysis of Jun D expression in non-transfected: (A) and mouse Jun D transfected: (B) 293T whole cell lysates and NIH/3T3 nuclear extract (C).Application
Immunofluorescence staining of normal mouse intestine frozen section showing nuclear staining.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human adrenal gland tissue showing nuclear staining of cortical cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of Jun D expression in untreated (A) and phorbol ester-induced (B) NIH/3T3 nuclear extracts.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human colon carcinoma tissue showing nuclear staining.| Product Name | Jun D Antibody (329) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of Jun D of mouse origin |
| Modification Notes | The activator protein-1 (AP-1) transcription factor consists of either Jun/Jun homodimers or Fos/Jun heterodimeric complexes. Homo- and heterodimers bind to the TGACTCA consensus sequence present in numerous promoters and initially identified as the phorbol ester tumor promoter response element (TRE). Jun B and Jun D have been shown to be almost identical to c-Jun in their C-terminal regions, which are involved in dimerization and DNA binding, whereas their N-terminal domains, which are involved in transcriptional activation, diverge. All three form heterodimers among themselves and with c-Fos and other members of the Fos gene family. Studies suggest that the two forms of Jun D may be due to internal initiation of translation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of Jun D of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of Jun D of mouse origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of Jun D of mouse origin |
| Entrez Gene | 3727 |
|---|
Application
Western blot analysis of Jun D expression in non-transfected: (A) and mouse Jun D transfected: (B) 293T whole cell lysates and NIH/3T3 nuclear extract (C).
Application
Immunofluorescence staining of normal mouse intestine frozen section showing nuclear staining.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human adrenal gland tissue showing nuclear staining of cortical cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of Jun D expression in untreated (A) and phorbol ester-induced (B) NIH/3T3 nuclear extracts.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human colon carcinoma tissue showing nuclear staining.| Application Notes | recommended for detection of Jun D of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
