Application
Western blot analysis of 14-3-3 θ expression in non-transfected 293T: (A), human 14-3-3 θ transfected 293T: (B) and PC-12 (C) whole cell lysates and mouse placenta tissue extract (D).Application
Western blot analysis of 14-3-3 ζ expression in non-transfected: (A) and human 14-3-3 ζ transfected: (B) 293 whole cell lysates.Application
Western blot analysis of 14-3-3 σ expression in non-transfected 293T: (A), human 14-3-3 σ transfected 293T (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of 14-3-3 γ expression in non-transfected 293T: (A), human 14-3-3 γ transfected 293T: (B) and A-431 (C) whole cell lysates.Application
Western blot analysis of 14-3-3 η expression in non-transfected 293T: (A), mouse 14-3-3 η transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.Application
Western blot analysis of 14-3-3 θ expression in non-transfected 293T: (A), human 14-3-3 θ transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.Application
Western blot analysis of 14-3-3 ζ expression in non-transfected 293T: (A), human 14-3-3 ζ transfected 293T: (B) and KNRK (C) whole cell lysates.Application
Western blot analysis of pan 14-3-3 θ non-transfected 293T: (A), human pan 14-3-3 θ transfected 293T: (B) and NIH/3T3 (C) whole cell lysate.Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization using indirect FITC (A) staining and HeLA cells using direct Alexa Fluor 488 (B) staining.Application
Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal rabbit IgG: .Application
Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal rabbit IgG: .Application
Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal rabbit IgG: .Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human liver tumor showing cytoplasmic staining of hepatocytes.| Product Name | pan 14-3-3 Antibody (K-19) AC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the N-terminus of 14-3-3 β of human origin |
| Modification Notes | 14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 ∫, ©, é, Ω, ñ, † and s. 14-3-3 proteins form dimers that present two binding sites for ligand proteins, thereby bringing together two proteins that may not otherwise associate. These ligands largely share a 14-3-3 consensus binding motif and exhibit serine/threonine phosphorylation. 14-3-3 proteins function in broad regulation of these ligand proteins, by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, activation/repression of enzymatic activity and facilitation of protein modification, and thus loss of expression contributes to a vast array of pathogenic cellular activities. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCFCELISA |
| recommended for detection of members of the 14-3-3 protein family of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the N-terminus of 14-3-3 &beta of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the N-terminus of 14-3-3 β of human origin |
| Entrez Gene | 7529 |
|---|
Application
Western blot analysis of 14-3-3 θ expression in non-transfected 293T: (A), human 14-3-3 θ transfected 293T: (B) and PC-12 (C) whole cell lysates and mouse placenta tissue extract (D).
Application
Western blot analysis of 14-3-3 ζ expression in non-transfected: (A) and human 14-3-3 ζ transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of 14-3-3 σ expression in non-transfected 293T: (A), human 14-3-3 σ transfected 293T (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of 14-3-3 γ expression in non-transfected 293T: (A), human 14-3-3 γ transfected 293T: (B) and A-431 (C) whole cell lysates.
Application
Western blot analysis of 14-3-3 η expression in non-transfected 293T: (A), mouse 14-3-3 η transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.
Application
Western blot analysis of 14-3-3 θ expression in non-transfected 293T: (A), human 14-3-3 θ transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.
Application
Western blot analysis of 14-3-3 ζ expression in non-transfected 293T: (A), human 14-3-3 ζ transfected 293T: (B) and KNRK (C) whole cell lysates.
Application
Western blot analysis of pan 14-3-3 θ non-transfected 293T: (A), human pan 14-3-3 θ transfected 293T: (B) and NIH/3T3 (C) whole cell lysate.
Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization using indirect FITC (A) staining and HeLA cells using direct Alexa Fluor 488 (B) staining.
Application
Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human liver tumor showing cytoplasmic staining of hepatocytes.| Application Notes | recommended for detection of members of the 14-3-3 protein family of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
