Application
Western blot analysis of MyoD expression in L8 (A), A-673 (B), Sol8 (C) and C2C12 (D) whole cell lysates and mouse skeletal muscle tissue extract (E) and mouse recombinant MyoD fusion protein (F).Application
Western blot analysis of MyoD expression in L8 whole cell lysate.| Product Name | MyoD Antibody (M-318) AC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-318 representing full length MyoD protein of mouse origin |
| Modification Notes | Differentiation of myogenic cells is regulated by multiple positively and negatively acting factors. One well characterized family of helix-loop-helix (HLH) proteins known to play an important role in the regulation of muscle cell development includes MyoD, myogenin, Myf-5 and Myf-6 (also designated MRF-4 or herculin). Of interest, most muscle cells express either MyoD or Myf-5 in the committed state, but when induced to differentiate, all turn on expression of myogenin. MyoD transcription factors form heterodimers with products of a more widely expressed family of bHLH genes, the E family, which consists of at least three distinct genes: E2A, IF2 and HEB. MyoD-E heterodimers bind avidly to consensus (CANNTG) E box target sites that are functionally important elements in the upstream regulatory sequences of many muscle-specific terminal differentiation genes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of MyoD of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-318 representing full length MyoD protein of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-318 representing full length MyoD protein of mouse origin |
| Entrez Gene | 4654 |
|---|
Application
Western blot analysis of MyoD expression in L8 (A), A-673 (B), Sol8 (C) and C2C12 (D) whole cell lysates and mouse skeletal muscle tissue extract (E) and mouse recombinant MyoD fusion protein (F).
Application
Western blot analysis of MyoD expression in L8 whole cell lysate.| Application Notes | recommended for detection of MyoD of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
