Application
Western blot analysis of Cdk6 expression in HL-60 (A) and HT-1080 (B) whole cell lysates.Application
Western blot analysis of Cdk6 expression in K-562 whole cell lysate.Application
Western blot analysis of Cdk6 expression in non-transfected 293T: (A), human Cdk6 transfected 293T: (B) and K-562 (C) whole cell lysates.Application
Western blot analysis of Cdk6 expression in non-transfected 293T: (A), human Cdk6 transfected 293T: (B) and Jurkat (C) whole cell lysates.Application
Indirect FCM analysis of methanol permeabilized K-562 cells stained with Cdk6 (C-21), followed by PE-conjugated goat anti-rabbit IgG: .Application
Western blot analysis of Cdk6 expression in non-transfected control (A) and Cdk6 siRNA transfected (B) HeLa cells. Blot probed with Cdk6 (C-21): . Actin (I-19): used as specificity and loading control.Application
Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear staining.Application
Western blot analysis of Cdk6 expression in K-562 (A) and Jurkat (B) whole cell lysates.| Product Name | Cdk6 Antibody (C-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of Cdk6 of human origin |
| Modification Notes | Cell cycle progression is controlled in part by a family of cyclin proteins and cyclin dependent kinases (Cdks). Cdk proteins work in concert with the cyclins to phosphorylate key substrates involved in each phase of cell cycle progression. Another family of proteins, Cdk inhibitors, also plays a role in regulating the cell cycle by binding to cyclin-Cdk complexes and modulating their activity. Several Cdk proteins have been identified, including Cdk2-Cdk8, PCTAIRE-1-PCTAIRE-3, PITALRE and PITSLRE. Cdk6 is known to associate with cyclins D1, D2 and D3 and to be involved with the G1/S transition of the cell cycle. Multiple inhibitors of Cdk6 have been identified, including p18 and p19. These inhibitors bind to both free and complexed Cdk6, and they inhibit the activity of the cyclin D-bound Cdk6. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFFCELISA |
| recommended for detection of Cdk6 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF, ELISA and indirect FCM using PE-conjugated goat anti-rabbit IgG: sc-3739 also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of Cdk6 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of Cdk6 of human origin |
| Entrez Gene | 1021 |
|---|
Application
Western blot analysis of Cdk6 expression in HL-60 (A) and HT-1080 (B) whole cell lysates.
Application
Western blot analysis of Cdk6 expression in K-562 whole cell lysate.
Application
Western blot analysis of Cdk6 expression in non-transfected 293T: (A), human Cdk6 transfected 293T: (B) and K-562 (C) whole cell lysates.
Application
Western blot analysis of Cdk6 expression in non-transfected 293T: (A), human Cdk6 transfected 293T: (B) and Jurkat (C) whole cell lysates.
Application
Indirect FCM analysis of methanol permeabilized K-562 cells stained with Cdk6 (C-21), followed by PE-conjugated goat anti-rabbit IgG: .
Application
Western blot analysis of Cdk6 expression in non-transfected control (A) and Cdk6 siRNA transfected (B) HeLa cells. Blot probed with Cdk6 (C-21): . Actin (I-19): used as specificity and loading control.
Application
Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear staining.
Application
Western blot analysis of Cdk6 expression in K-562 (A) and Jurkat (B) whole cell lysates.| Application Notes | recommended for detection of Cdk6 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF, ELISA and indirect FCM using PE-conjugated goat anti-rabbit IgG: sc-3739 also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
