Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.Application
Western blot analysis of Cdk2 expression in non-transfected: (A) and human Cdk2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Cdk2 expression in non-transfected: (A) and human Cdk2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Cdk2 expression in non-transfected: (A) and mouse Cdk2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Cdk2 expression in NAMALWA whole cell lysate.| Product Name | Cdk2 Antibody (H-298) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-298 representing full length Cdk2 of human origin |
| Modification Notes | In vertebrates, as in yeast, multiple cyclins have been identified, including a total of eight such regulatory proteins in mammals. In contrast to the situation in yeast, the Cdc2 p34 kinase is not the only catalytic subunit identified in vertebrates that can interact with cyclins. While Cdc2 p34 is essential for the G2 to M transition in vertebrate cells, a second Cdc2-related kinase has also been implicated in cell cycle control. This protein, designated cyclin-dependent kinase 2 (Cdk2) p33, also binds to cyclins and its kinase activity is temporally regulated during the cell cycle. Several additional Cdc2 p34-related cyclin dependent kinases have been identified. These include Cdk3-Cdk8, PCTAIRE-1-3 and KKIALRE. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Cdk2 of mouse, rat and human origin by WB, IP, IF and ELISA minimal cross-reactivity with other cyclin dependent kinases also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-298 representing full length Cdk2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-298 representing full length Cdk2 of human origin |
| Entrez Gene | 1017 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Application
Western blot analysis of Cdk2 expression in non-transfected: (A) and human Cdk2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Cdk2 expression in non-transfected: (A) and human Cdk2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Cdk2 expression in non-transfected: (A) and mouse Cdk2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Cdk2 expression in NAMALWA whole cell lysate.| Application Notes | recommended for detection of Cdk2 of mouse, rat and human origin by WB, IP, IF and ELISA minimal cross-reactivity with other cyclin dependent kinases also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
