Application
Western blot analysis of Rb expression in K-562 whole cell lysate.Application
Western blot analysis of Rb expression in non-transfected 293T: (A), human Rb transfected 293T: (B) and K-562 (C) whole cell lysates.Application
Western blot analysis of Rb expression in non-transfected: (A) and human Rb transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Rb expression in non-transfected 293T: (A) and human Rb transfected 293T: (B) 29.3T whole cell lysates.Application
Antibodies tested include Rb (C-15): (A), Rb (C-15)-G: (B), Rb (IF8): (C) and Rb (M-153): (D).Application
Nuclear immunofluorescence staining of methanol-fixed NIH/3T3 mouse cells.| Product Name | Rb Antibody (C-15) TRITC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of Rb of human origin |
| Modification Notes | Pediatric cancer retinoblastoma and the formation of other human tumors can be attributed to mutations in the retinoblastoma tumor suppressor gene. The retinoblastoma tumor suppressor gene product, known as Rb or pRb, regulates differentiation, apoptosis and cell cycle control by coordinating the cell cycle, at G1/S, with transcriptional machinery that includes the heterodimeric E2F family. During G1, cyclin D (D1, D2, D3)-dependent kinase-mediated phosphorylation of Rb at Ser 795 marks the conversion of Rb from a transcriptionally repressive, hypophosphorylated state to an inactive, phosphorylated state, which may be sustained through mitosis by differential phosphorylation of up to 16 putative serine or threonine residues, including Ser 249/Thr 252, Thr 373, Thr 356, Ser 780, Ser 807/Ser 811 and Thr 821/Thr 826. Hypophosphorylated Rb represses the transcription of genes controlling cell cycle through direct protein-protein interactions, by binding and inactivating the promoters of transcription factors, and through recruitment of histone deacetylase, which deacetylates promoter regions and enhances nucleosome formation, thereby masking transcription enhancing cis elements. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of Rb p110 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of Rb of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of Rb of human origin |
| Entrez Gene | 5925 |
|---|
Application
Western blot analysis of Rb expression in K-562 whole cell lysate.
Application
Western blot analysis of Rb expression in non-transfected 293T: (A), human Rb transfected 293T: (B) and K-562 (C) whole cell lysates.
Application
Western blot analysis of Rb expression in non-transfected: (A) and human Rb transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Rb expression in non-transfected 293T: (A) and human Rb transfected 293T: (B) 29.3T whole cell lysates.
Application
Antibodies tested include Rb (C-15): (A), Rb (C-15)-G: (B), Rb (IF8): (C) and Rb (M-153): (D).
Application
Nuclear immunofluorescence staining of methanol-fixed NIH/3T3 mouse cells.| Application Notes | recommended for detection of Rb p110 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
