Application
Western blot analysis of Cdk4 expression in HeLa whole cell lysate. Antibody tested: Cdk4 (H-22)-G: .Application
Western blot analysis of Cdk4 expression in Ramos whole cell lysate.Application
Western blot analysis of Cdk4 expression in non-transfected 293T: (A), mouse Cdk4 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear staining.Application
Western blot analysis of Cdk4 expression in HeLa (A), Ramos (B) and K-562 (C) whole cell lysates.| Product Name | Cdk4 Antibody (H-22) AC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of Cdk4 of human origin |
| Modification Notes | Cell cycle progression is controlled in part by a family of cyclin proteins and cyclin dependent kinases (Cdks). Cdk proteins work in concert with the cyclins to phosphorylate key substrates involved in each phase of cell cycle progression. Another family of proteins, Cdk inhibitors, also plays a role in regulating the cell cycle by binding to cyclin-Cdk complexes and modulating their activity. Several Cdk proteins have been identified, including Cdk2-Cdk8, PCTAIRE-1-PCTAIRE-3, PITALRE and PITSLRE. Cdk4, in complex with D-type cyclins, is thought to regulate cell growth during the G1 phase of the cell cycle. This association with a D-type cyclin upregulates Cdk4 activity, whereas binding to the Cdk inhibitor p16 downregulates Cdk4 activity. Activation of the Cdk4-cyclin complexes requires phosphorylation on a single threonyl residue of Cdk4, catalyzed by a Cdk-activating protein (CAK). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Cdk4 of mouse, rat and human origin by WB, IP, KA, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of Cdk4 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of Cdk4 of human origin |
| Entrez Gene | 1019 |
|---|
Application
Western blot analysis of Cdk4 expression in HeLa whole cell lysate. Antibody tested: Cdk4 (H-22)-G: .
Application
Western blot analysis of Cdk4 expression in Ramos whole cell lysate.
Application
Western blot analysis of Cdk4 expression in non-transfected 293T: (A), mouse Cdk4 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear staining.
Application
Western blot analysis of Cdk4 expression in HeLa (A), Ramos (B) and K-562 (C) whole cell lysates.| Application Notes | recommended for detection of Cdk4 of mouse, rat and human origin by WB, IP, KA, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
