Application
Western blot analysis of Nlk expression in non-transfected: (A) and mouse Nlk transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Nlk expression in non-transfected: (A) and human Nlk transfected: (B) 293T whole cell lysates.Application
Western blot analysis of human recombinant Nlk.| Product Name | Nlk Antibody (H-100) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 416-515 mapping at the C-terminus of Nlk of human origin |
| Modification Notes | The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2 (1-4), respectively. ERK proteins are regulated by dual phosphorylation at specific tyrosine and threonine sites mapping within a characteristic Thr-Glu-Tyr motif (5,6). Phosphorylation at both Thr-183 and Tyr-185 is required for full enzymatic activation (5,6). In response to activation, MAP kinases phosphorylate downstream components on serine and threonine (5,6). Nlk, or nemo-like kinase, is a murine homolog of the Drosophila nemo (nmo) gene (7). Nlk and Nmo have sequence homology to both the ERK MAP kinases and the cyclin dependent kinases. Nlk is a nuclear protein with the ability to autophosphorylate (7). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Nlk of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 416-515 mapping at the C-terminus of Nlk of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 416-515 mapping at the C-terminus of Nlk of human origin |
| Entrez Gene | 51701 |
|---|
Application
Western blot analysis of Nlk expression in non-transfected: (A) and mouse Nlk transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Nlk expression in non-transfected: (A) and human Nlk transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of human recombinant Nlk.| Application Notes | recommended for detection of Nlk of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
