Application
Western blot analysis of R1 expression in HeLa (A) and MCF7 (B) whole cell lysates.Application
Western blot analysis of R1 expression in Ramos whole cell lysate.Application
Immuno?fluorescence staining of methanol-fixed HeLa cells showing cytoplasmic local?ization.| Product Name | R1 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 of R1 of human origin |
| Modification Notes | Ribonucleotide reductase is essential for the production and maintenance of the level of deoxyribonucleoside triphosphates (dNTP’s) required for DNA synthesis. It is an enzymatic complex consisting of two nonidentical subunits, R1 and R2, which are inactive separately. R1, the larger subunit, contains allosteric regulatory sites in a human breast carcinoma cell line. R2 is the limiting factor of the catalytic activity of the ribonucleotide reductase enzymatic complex. R2 expression is strictly correlated to the S-phase of the cell cycle, whereas R1 remains constant throughout all phases of the cell cycle. Ribonucleotide reductase appears to be specifically involved in nucleotide excision repair, since both the R1 and R2 subunits are induced in response to UV light in a dose-dependent manner. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of R1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 of R1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 of R1 of human origin |
| Entrez Gene | 6240 |
|---|
Application
Western blot analysis of R1 expression in HeLa (A) and MCF7 (B) whole cell lysates.
Application
Western blot analysis of R1 expression in Ramos whole cell lysate.
Application
Immuno?fluorescence staining of methanol-fixed HeLa cells showing cytoplasmic local?ization.| Application Notes | recommended for detection of R1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
