Application
Western blot analysis of cleaved MEK kinase-1 expression in HL-60 whole cell lysate.Application
Antibodies tested include MEK-1 (C-18): , MEK kinase-1 (C-22): , HDAC1 (C-19): , HDAC1 (H-11): and HDAC1 (H-11): . Data kindly provided by M.G. Rosenfeld and reproduced with permission from Baek et al., Cell 2002, 110: 55-67.Application
Western blot analysis of cleaved MEK kinase-1 expression in A-431 whole cell lysate.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse lymph node showing cytoplasmic localization.| Product Name | MEK kinase-1 Antibody (C-22) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of MEK kinase-1 of rat origin |
| Modification Notes | Mitogen-activated protein (MAP) kinase cascades are activated by various extracellular stimuli including growth factors. The MEK kinases (also designated MAP kinase kinase kinases, MKKKs, MAP3Ks or MEKKs) phosphorylate and thereby activate the MEKs (also called MAP kinase kinases or MKKs), including ERK, JNK and p38. These activated MEKs in turn phosphorylate and activate the MAP kinases. The MEK kinases include Raf-1, Raf-B, Mos, MEK kinase-1, MEK kinase-2, MEK kinase-3, MEK kinase-4, ASK 1 (MEK kinase-5) and MAP3K6 (MEK kinase-6). MEK kinase-1 activates the ERK and c-Jun NH(2)-terminal kinase (JNK) pathways by phosphorylation of MAP2K1 and MAP2K4, and also activates the central protein kinases of the NF˚B pathway, CHUK and IKBKB. Additionally, MEK kinase-1 uses an E3 ligase through its PHD domain, a RING-finger-like structure, to target proteins for degradation through ubiquitination. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAChIP |
| recommended for detection of MEK kinase-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of MEK kinase-1 of rat origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of MEK kinase-1 of rat origin |
| Entrez Gene | 4214 |
|---|
Application
Western blot analysis of cleaved MEK kinase-1 expression in HL-60 whole cell lysate.
Application
Antibodies tested include MEK-1 (C-18): , MEK kinase-1 (C-22): , HDAC1 (C-19): , HDAC1 (H-11): and HDAC1 (H-11): . Data kindly provided by M.G. Rosenfeld and reproduced with permission from Baek et al., Cell 2002, 110: 55-67.
Application
Western blot analysis of cleaved MEK kinase-1 expression in A-431 whole cell lysate.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse lymph node showing cytoplasmic localization.| Application Notes | recommended for detection of MEK kinase-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
