Application
Western blot analysis of Crk II expression in non-transfected: (A) and human Crk II transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Crk II expression in non-transfected: (A) and mouse Crk II transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Crk II expression in non-transfected: (A) and human Crk II transfected: (B) 293 whole cell lysates.Application
Immunofluorescence staining of normal mouse intestine frozen section showing membrane staining.Application
Western blot analysis of Crk II expression in HeLa (A), K-562 (B), NIH/3T3 (C) and Rat 1 Rin (D) whole cell lysates.| Product Name | Crk II Antibody (C-18) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of Crk II of human origin |
| Modification Notes | The Crk family of adapter proteins including Crk-II, Crk-I, and Crk-L consist mostly of SH2 and SH3 domains (1). Through the interactions between SH2 domain and phosphotyrosine residues and/or between SH3 domain and proline-rich motifs, they are involved in a variety of signaling cascades (1). Crk I and Crk II are encoded by the same gene, which undergoes alternative splicing to yield these two proteins, but differ in their biological activities (2-4). Crk-II has less transforming activity than Crk-I, although both Crk-I and Crk-II bind to many tyrosine-phosphorylated proteins that bind to grb2 (2,4). In addition, Crk-II becomes rapidly tyrosine-phosphorylated in response to stimulation with insulin-like growth factor I (IGF-I) and might be involved in the IGF-I receptor signalling pathway (5). The gene encoding Crk I and II maps to human chromosome 17p13, a region which demonstrates frequent deletion or loss of heterozygosity in a wide range of human cancers (3). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Crk II p40 and p42 of mouse, rat, human and avian origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of Crk II of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of Crk II of human origin |
| Entrez Gene | 1398 |
|---|
Application
Western blot analysis of Crk II expression in non-transfected: (A) and human Crk II transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Crk II expression in non-transfected: (A) and mouse Crk II transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Crk II expression in non-transfected: (A) and human Crk II transfected: (B) 293 whole cell lysates.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing membrane staining.
Application
Western blot analysis of Crk II expression in HeLa (A), K-562 (B), NIH/3T3 (C) and Rat 1 Rin (D) whole cell lysates.| Application Notes | recommended for detection of Crk II p40 and p42 of mouse, rat, human and avian origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
