Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing nuclear staining of Islets of Langerhans and glandular cells.Application
Western blot analysis of TIF1β expression in non-transfected 293T: (A), human TIF1β transfected 293T: (B) and CCRF-CEM (C) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing nuclear staining of trophoblastic cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of normal mouse intestine frozen section showing nuclear staining.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.Application
Western blot analysis of TIF1β expression in CCRF-CEM nuclear extract.| Product Name | TIF1β Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 536-835 mapping at the C-terminus of TIF1β of human origin |
| Modification Notes | TIF1∫, for transcriptional intermediary factor 1-beta, also designated KAP1(for KRAB-associated protein 1), TF1∫ and TRIM28 (for tripartif motif-containing 28), is a member of the tripartif motif family characterized by three zinc-binding domains, a RING finger, B-boxes and a coiled-coil domain (1,2,4). Like TIF1å, TIF1∫ contains both a Cys/His PHD (plant homeodomain) finger and bromodomain that form a cooperative unit required for transcriptional repression (3,5). TIF1∫ mediates transcriptional control by interaction with the Kruppel-associated box (KRAB) repression domain found in many transcription factors and by binding DNA through its zinc finger (3,6). The human TIF1∫ gene maps to human chromosome 19q13.4 and encodes an 835 amino acid nuclear protein (3,4). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of TIF1β of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 536-835 mapping at the C-terminus of TIF1&beta of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 536-835 mapping at the C-terminus of TIF1β of human origin |
| Entrez Gene | 10155 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing nuclear staining of Islets of Langerhans and glandular cells.
Application
Western blot analysis of TIF1β expression in non-transfected 293T: (A), human TIF1β transfected 293T: (B) and CCRF-CEM (C) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing nuclear staining of trophoblastic cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing nuclear staining.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Application
Western blot analysis of TIF1β expression in CCRF-CEM nuclear extract.| Application Notes | recommended for detection of TIF1β of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
