Application
Western blot analysis of Ect2 expression in non-transfected: (A) and mouse Ect2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Ect2 expression in non-transfected: (A) and mouse Ect2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Ect2 expression in HeLa whole cell lysates.Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing nuclear staining.Application
Western blot analysis of Ect2 expression in NIH/3T3 whole cell lysate.| Product Name | Ect2 Antibody (C-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of Ect2 of mouse origin |
| Modification Notes | Numerous cellular functions such as proliferation, differentiation, apoptosis, vesicular trafficking, nuclear transport and cytoskeletal organization are controlled by GTPases (1,2). It has become increasingly clear that GTPases act in cascades in which their activities are linked by GTPase-activating proteins (GAPs) and guanine nucleotide exchange factors (GEFs) (1,2). In a search for new epithelial cell-specific oncogenes using a highly efficient cDNA expression cloning system (3), the ost oncogene was isolated from rat osteosarcoma cells (4). The Ost proto-oncogene protein contains DH (5,6) and PH (7) domains and catalyzes guanine nucleotide exchange on RhoA and Cdc42 and interacts specifically with the GTP-bound form of Rac1. A similar protein, Ect2, specifically interacts with Rho and Rac proteins in vitro. Ect2 shares sequence homology with the 255 amino acid central core of the breakpoint cluster gene, bcr, as well as with yeast CDC24 and the Dbl oncogene, all of which have been shown to modulate the function of small Rho-like GTP binding proteins (8). The Ect2 contains both PH and DH domains (8). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Ect2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of Ect2 of mouse origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of Ect2 of mouse origin |
| Entrez Gene | 1894 |
|---|
Application
Western blot analysis of Ect2 expression in non-transfected: (A) and mouse Ect2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Ect2 expression in non-transfected: (A) and mouse Ect2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Ect2 expression in HeLa whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing nuclear staining.
Application
Western blot analysis of Ect2 expression in NIH/3T3 whole cell lysate.| Application Notes | recommended for detection of Ect2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
